Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS

T. T N Lien; Tran Dai Lam; V. T H An; Tran Vinh Hoang; Duong Tuan Quang; Dinh Quang Khieu; Toshifumi Tsukahara; Young Hoon Lee; Jong Seung Kim

doi:10.1016/j.talanta.2009.09.002

Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS

T. T N Lien, Tran Dai Lam, V. T H An, Tran Vinh Hoang, Duong Tuan Quang, Dinh Quang Khieu, Toshifumi Tsukahara, Young Hoon Lee, Jong Seung Kim

Research output: Contribution to journal › Article › peer-review

80 Citations (Scopus)

Abstract

In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (R_ct) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4 pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.

Original language	English
Pages (from-to)	1164-1169
Number of pages	6
Journal	Talanta
Volume	80
Issue number	3
DOIs	https://doi.org/10.1016/j.talanta.2009.09.002
Publication status	Published - 2010 Jan

Bibliographical note

Funding Information:
T.T.N. Lien wishes to thank the receipt of a grant from the Flemish Interuniversity Council for University Development cooperation (VLIR UOS), V.T.H. An would like to acknowledge HUT university grant T2008-01 . J.S. Kim is grateful for grant of Creative Research Initiative program supported by MEST.

Keywords

Electrochemical DNA biosensor
Electrochemical impedance spectroscopy
MWCNTs-doped polypyrrole
Quartz crystal microbalance

ASJC Scopus subject areas

Analytical Chemistry

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10.1016/j.talanta.2009.09.002

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title = "Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS",

abstract = "In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (Rct) and signifying {"}signal-on{"} behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4 pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.",

keywords = "Electrochemical DNA biosensor, Electrochemical impedance spectroscopy, MWCNTs-doped polypyrrole, Quartz crystal microbalance",

author = "Lien, {T. T N} and Lam, {Tran Dai} and An, {V. T H} and Hoang, {Tran Vinh} and Quang, {Duong Tuan} and Khieu, {Dinh Quang} and Toshifumi Tsukahara and Lee, {Young Hoon} and Kim, {Jong Seung}",

note = "Funding Information: T.T.N. Lien wishes to thank the receipt of a grant from the Flemish Interuniversity Council for University Development cooperation (VLIR UOS), V.T.H. An would like to acknowledge HUT university grant T2008-01 . J.S. Kim is grateful for grant of Creative Research Initiative program supported by MEST. ",

year = "2010",

month = jan,

doi = "10.1016/j.talanta.2009.09.002",

language = "English",

volume = "80",

pages = "1164--1169",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier B.V.",

number = "3",

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T1 - Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS

AU - Lien, T. T N

AU - Lam, Tran Dai

AU - An, V. T H

AU - Hoang, Tran Vinh

AU - Quang, Duong Tuan

AU - Khieu, Dinh Quang

AU - Tsukahara, Toshifumi

AU - Lee, Young Hoon

AU - Kim, Jong Seung

N1 - Funding Information: T.T.N. Lien wishes to thank the receipt of a grant from the Flemish Interuniversity Council for University Development cooperation (VLIR UOS), V.T.H. An would like to acknowledge HUT university grant T2008-01 . J.S. Kim is grateful for grant of Creative Research Initiative program supported by MEST.

PY - 2010/1

Y1 - 2010/1

N2 - In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (Rct) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4 pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.

AB - In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (Rct) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4 pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.

KW - Electrochemical DNA biosensor

KW - Electrochemical impedance spectroscopy

KW - MWCNTs-doped polypyrrole

KW - Quartz crystal microbalance

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Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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