Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America

Young Woon Lim; Sepideh Massoumi Alamouti; Jae Jin Kim; Sangwon Lee; Colette Breuil

doi:10.1016/j.femsle.2004.06.020

Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America

Young Woon Lim, Sepideh Massoumi Alamouti, Jae Jin Kim, Sangwon Lee, Colette Breuil

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Leptographium pyrinum, Leptographium terebrantis, Ophiostoma aureum, Ophiostoma clavigerum, and Ophiostoma robustum are very similar in morphology, host trees choice, and the way they are disseminated by bark beetles. Their phylogenetic relationships were clarified using rDNA and protein coding genes including actin, β-tubulin, and translation elongation factor-1α. Protein coding gene trees showed better resolution than the rDNA tree, which generated three clades: O. clavigerum, L. terebrantis/L. pyrinum, and O. robustum/O. aureum. A combined gene phylogenetic tree, which was supported by high bootstrap values, showed that O. aureum, L. pyrinum, O. robustum, and O. clavigerum each formed distinct clades while L. terebrantis was paraphyletic to O. clavigerum. The higher variability of the protein coding genes and the congruity in their phylogenetic results suggested that these genes may be better markers for identifying closely related species. These gene trees have also facilitated the description of the evolutionary relationships among these species.

Original language	English
Pages (from-to)	89-96
Number of pages	8
Journal	FEMS microbiology letters
Volume	237
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2004.06.020
Publication status	Published - 2004 Aug 1
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Natural Sciences and Engineering Research Council of Canada. We thank Drs. D.L. Six, M.J. Wingfield, and A. Uzunovic for providing cultures. We thank Julie Yi (Macrogen) and Monica Lu for technical assistance.

Keywords

Actin
Bark beetle
Multigene phylogenies
Ophiostoma clavigerum
Translation elongation factor 1α
rDNA
β-Tubulin

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/j.femsle.2004.06.020

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title = "Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America",

abstract = "Leptographium pyrinum, Leptographium terebrantis, Ophiostoma aureum, Ophiostoma clavigerum, and Ophiostoma robustum are very similar in morphology, host trees choice, and the way they are disseminated by bark beetles. Their phylogenetic relationships were clarified using rDNA and protein coding genes including actin, β-tubulin, and translation elongation factor-1α. Protein coding gene trees showed better resolution than the rDNA tree, which generated three clades: O. clavigerum, L. terebrantis/L. pyrinum, and O. robustum/O. aureum. A combined gene phylogenetic tree, which was supported by high bootstrap values, showed that O. aureum, L. pyrinum, O. robustum, and O. clavigerum each formed distinct clades while L. terebrantis was paraphyletic to O. clavigerum. The higher variability of the protein coding genes and the congruity in their phylogenetic results suggested that these genes may be better markers for identifying closely related species. These gene trees have also facilitated the description of the evolutionary relationships among these species.",

keywords = "Actin, Bark beetle, Multigene phylogenies, Ophiostoma clavigerum, Translation elongation factor 1α, rDNA, β-Tubulin",

author = "Lim, {Young Woon} and Alamouti, {Sepideh Massoumi} and Kim, {Jae Jin} and Sangwon Lee and Colette Breuil",

note = "Funding Information: This work was supported by the Natural Sciences and Engineering Research Council of Canada. We thank Drs. D.L. Six, M.J. Wingfield, and A. Uzunovic for providing cultures. We thank Julie Yi (Macrogen) and Monica Lu for technical assistance.",

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AU - Lee, Sangwon

AU - Breuil, Colette

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N2 - Leptographium pyrinum, Leptographium terebrantis, Ophiostoma aureum, Ophiostoma clavigerum, and Ophiostoma robustum are very similar in morphology, host trees choice, and the way they are disseminated by bark beetles. Their phylogenetic relationships were clarified using rDNA and protein coding genes including actin, β-tubulin, and translation elongation factor-1α. Protein coding gene trees showed better resolution than the rDNA tree, which generated three clades: O. clavigerum, L. terebrantis/L. pyrinum, and O. robustum/O. aureum. A combined gene phylogenetic tree, which was supported by high bootstrap values, showed that O. aureum, L. pyrinum, O. robustum, and O. clavigerum each formed distinct clades while L. terebrantis was paraphyletic to O. clavigerum. The higher variability of the protein coding genes and the congruity in their phylogenetic results suggested that these genes may be better markers for identifying closely related species. These gene trees have also facilitated the description of the evolutionary relationships among these species.

AB - Leptographium pyrinum, Leptographium terebrantis, Ophiostoma aureum, Ophiostoma clavigerum, and Ophiostoma robustum are very similar in morphology, host trees choice, and the way they are disseminated by bark beetles. Their phylogenetic relationships were clarified using rDNA and protein coding genes including actin, β-tubulin, and translation elongation factor-1α. Protein coding gene trees showed better resolution than the rDNA tree, which generated three clades: O. clavigerum, L. terebrantis/L. pyrinum, and O. robustum/O. aureum. A combined gene phylogenetic tree, which was supported by high bootstrap values, showed that O. aureum, L. pyrinum, O. robustum, and O. clavigerum each formed distinct clades while L. terebrantis was paraphyletic to O. clavigerum. The higher variability of the protein coding genes and the congruity in their phylogenetic results suggested that these genes may be better markers for identifying closely related species. These gene trees have also facilitated the description of the evolutionary relationships among these species.

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Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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