Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America

Young Woon Lim, Sepideh Massoumi Alamouti, Jae Jin Kim, Sangwon Lee, Colette Breuil

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


Leptographium pyrinum, Leptographium terebrantis, Ophiostoma aureum, Ophiostoma clavigerum, and Ophiostoma robustum are very similar in morphology, host trees choice, and the way they are disseminated by bark beetles. Their phylogenetic relationships were clarified using rDNA and protein coding genes including actin, β-tubulin, and translation elongation factor-1α. Protein coding gene trees showed better resolution than the rDNA tree, which generated three clades: O. clavigerum, L. terebrantis/L. pyrinum, and O. robustum/O. aureum. A combined gene phylogenetic tree, which was supported by high bootstrap values, showed that O. aureum, L. pyrinum, O. robustum, and O. clavigerum each formed distinct clades while L. terebrantis was paraphyletic to O. clavigerum. The higher variability of the protein coding genes and the congruity in their phylogenetic results suggested that these genes may be better markers for identifying closely related species. These gene trees have also facilitated the description of the evolutionary relationships among these species.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalFEMS microbiology letters
Issue number1
Publication statusPublished - 2004 Aug 1
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the Natural Sciences and Engineering Research Council of Canada. We thank Drs. D.L. Six, M.J. Wingfield, and A. Uzunovic for providing cultures. We thank Julie Yi (Macrogen) and Monica Lu for technical assistance.


  • Actin
  • Bark beetle
  • Multigene phylogenies
  • Ophiostoma clavigerum
  • Translation elongation factor 1α
  • rDNA
  • β-Tubulin

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


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