Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher

Xinglu Huang; Magdalena Swierczewska; Ki Young Choi; Lei Zhu; Ashwinkumar Bhirde; Jinwoo Park; Kwangmeyung Kim; Jin Xie; Gang Niu; Kang Choon Lee; Seulki Lee; Xiaoyuan Chen

doi:10.1002/anie.201107795

Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher

Xinglu Huang
, Magdalena Swierczewska
, Ki Young Choi
, Lei Zhu
, Ashwinkumar Bhirde
, Jinwoo Park
, Kwangmeyung Kim
, Jin Xie
, Gang Niu
, Kang Choon Lee
, Seulki Lee^*
, Xiaoyuan Chen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

Abstract

Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).

Original language	English
Pages (from-to)	1625-1630
Number of pages	6
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	7
DOIs	https://doi.org/10.1002/anie.201107795
Publication status	Published - 2012 Feb 13
Externally published	Yes

Keywords

caspases
fluorescence
imaging agents
nanoparticles
proteolysis

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201107795

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abstract = "Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).",

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AU - Huang, Xinglu

AU - Swierczewska, Magdalena

AU - Choi, Ki Young

AU - Zhu, Lei

AU - Bhirde, Ashwinkumar

AU - Park, Jinwoo

AU - Kim, Kwangmeyung

AU - Xie, Jin

AU - Niu, Gang

AU - Lee, Kang Choon

AU - Lee, Seulki

AU - Chen, Xiaoyuan

PY - 2012/2/13

Y1 - 2012/2/13

N2 - Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).

AB - Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).

KW - caspases

KW - fluorescence

KW - imaging agents

KW - nanoparticles

KW - proteolysis

UR - https://www.scopus.com/pages/publications/84863044802

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DO - 10.1002/anie.201107795

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JF - Angewandte Chemie - International Edition

IS - 7

ER -

Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher

Abstract

Keywords

ASJC Scopus subject areas

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