Multiplex PCR−based next-generation sequencing and global diversity of seoul virus in humans and rats

Won Keun Kim; Jin Sun No; Seung Ho Lee; Dong Hyun Song; Daesang Lee; Jeong Ah Kim; Se Hun Gu; Sunhye Park; Seong Tae Jeong; Heung Chul Kim; Terry A. Klein; Michael R. Wiley; Gustavo Palacios; Jin Won Song

doi:10.3201/eid2402.171216

Multiplex PCR−based next-generation sequencing and global diversity of seoul virus in humans and rats

Won Keun Kim, Jin Sun No, Seung Ho Lee, Dong Hyun Song, Daesang Lee, Jeong Ah Kim, Se Hun Gu, Sunhye Park, Seong Tae Jeong, Heung Chul Kim, Terry A. Klein, Michael R. Wiley, Gustavo Palacios, Jin Won Song

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Seoul virus (SEOV) poses a worldwide public health threat. This virus, which is harbored by Rattus norvegicus and R. rattus rats, is the causative agent of hemorrhagic fever with renal syndrome (HFRS) in humans, which has been reported in Asia, Europe, the Americas, and Africa. Defining SEOV genome sequences plays a critical role in development of preventive and therapeutic strategies against the unique worldwide hantavirus. We applied multiplex PCR-based next-generation sequencing to obtain SEOV genome sequences from clinical and reservoir host specimens. Epidemiologic surveillance of R. norvegicus rats in South Korea during 2000-2016 demonstrated that the serologic prevalence of enzootic SEOV infections was not significant on the basis of sex, weight (age), and season. Viral loads of SEOV in rats showed wide dissemination in tissues and dynamic circulation among populations. Phylogenetic analyses showed the global diversity of SEOV and possible genomic configuration of genetic exchanges.

Original language	English
Pages (from-to)	249-257
Number of pages	9
Journal	Emerging Infectious Diseases
Volume	24
Issue number	2
DOIs	https://doi.org/10.3201/eid2402.171216
Publication status	Published - 2018 Feb

Bibliographical note

Publisher Copyright:
© 2018 Centers for Disease Control and Prevention (CDC). All rights reserved.

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3201/eid2402.171216

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title = "Multiplex PCR−based next-generation sequencing and global diversity of seoul virus in humans and rats",

abstract = "Seoul virus (SEOV) poses a worldwide public health threat. This virus, which is harbored by Rattus norvegicus and R. rattus rats, is the causative agent of hemorrhagic fever with renal syndrome (HFRS) in humans, which has been reported in Asia, Europe, the Americas, and Africa. Defining SEOV genome sequences plays a critical role in development of preventive and therapeutic strategies against the unique worldwide hantavirus. We applied multiplex PCR-based next-generation sequencing to obtain SEOV genome sequences from clinical and reservoir host specimens. Epidemiologic surveillance of R. norvegicus rats in South Korea during 2000-2016 demonstrated that the serologic prevalence of enzootic SEOV infections was not significant on the basis of sex, weight (age), and season. Viral loads of SEOV in rats showed wide dissemination in tissues and dynamic circulation among populations. Phylogenetic analyses showed the global diversity of SEOV and possible genomic configuration of genetic exchanges.",

author = "Kim, {Won Keun} and No, {Jin Sun} and Lee, {Seung Ho} and Song, {Dong Hyun} and Daesang Lee and Kim, {Jeong Ah} and Gu, {Se Hun} and Sunhye Park and Jeong, {Seong Tae} and Kim, {Heung Chul} and Klein, {Terry A.} and Wiley, {Michael R.} and Gustavo Palacios and Song, {Jin Won}",

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doi = "10.3201/eid2402.171216",

language = "English",

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AU - Lee, Daesang

AU - Kim, Jeong Ah

AU - Gu, Se Hun

AU - Park, Sunhye

AU - Jeong, Seong Tae

AU - Kim, Heung Chul

AU - Klein, Terry A.

AU - Wiley, Michael R.

AU - Palacios, Gustavo

AU - Song, Jin Won

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AB - Seoul virus (SEOV) poses a worldwide public health threat. This virus, which is harbored by Rattus norvegicus and R. rattus rats, is the causative agent of hemorrhagic fever with renal syndrome (HFRS) in humans, which has been reported in Asia, Europe, the Americas, and Africa. Defining SEOV genome sequences plays a critical role in development of preventive and therapeutic strategies against the unique worldwide hantavirus. We applied multiplex PCR-based next-generation sequencing to obtain SEOV genome sequences from clinical and reservoir host specimens. Epidemiologic surveillance of R. norvegicus rats in South Korea during 2000-2016 demonstrated that the serologic prevalence of enzootic SEOV infections was not significant on the basis of sex, weight (age), and season. Viral loads of SEOV in rats showed wide dissemination in tissues and dynamic circulation among populations. Phylogenetic analyses showed the global diversity of SEOV and possible genomic configuration of genetic exchanges.

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Multiplex PCR−based next-generation sequencing and global diversity of seoul virus in humans and rats

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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