TY - JOUR
T1 - Multiplex polymerase chain reaction assay for selective detection of Salmonella enterica serovar Typhimurium
AU - Lim, Young Hee
AU - Hirose, Kenji
AU - Izumiya, Hidemasa
AU - Arakawa, Eiji
AU - Takahashi, Hideyuki
AU - Terajima, Jun
AU - Itoh, Ken Ichiro
AU - Tamura, Kazumichi
AU - Kim, Sung Il
AU - Watanabe, Haruo
PY - 2003/8
Y1 - 2003/8
N2 - A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ,fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonella serovars, and 8 non-Salmonella enteric pathogens. Multiplex PCR proved to be capable of identifying S. enterica serovar Typhimurium specifically and differentiating it from other Salmonella serovars in addition to non-Salmonella enteric pathogens. Thus, this multiplex PCR assay can be practically applied to the identificaiton of S. enterica serovar Typhimurium.
AB - A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ,fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonella serovars, and 8 non-Salmonella enteric pathogens. Multiplex PCR proved to be capable of identifying S. enterica serovar Typhimurium specifically and differentiating it from other Salmonella serovars in addition to non-Salmonella enteric pathogens. Thus, this multiplex PCR assay can be practically applied to the identificaiton of S. enterica serovar Typhimurium.
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M3 - Article
C2 - 14583637
AN - SCOPUS:0345687387
SN - 1344-6304
VL - 56
SP - 151
EP - 155
JO - Japanese Journal of Infectious Diseases
JF - Japanese Journal of Infectious Diseases
IS - 4
ER -
