@inbook{717931a058f348959932547b79458c7b,
title = "Multiplexed detection of oligonucleotides with biobarcoded gold nanoparticle probes",
abstract = "Applications in a variety of fields rely on the high-throughput ultrasensitive and multiplexed detection of oligonucleotides. However, the conventional microarray-based techniques that employ fluorescent dyes are hampered by several limitations; they require target amplification, fluorophore labeling, and complicated instrumentation, while the fluorophore-labeled species themselves exhibit slow binding kinetics, photo-bleaching effects, and overlapping spectral profiles. Among the emerging nanomaterials that are being used to solve these problems, oligonucleotide-gold nanoparticle conjugates (Oligo-Au NPs) have recently been highlighted due to their unique chemical and physical properties. In this chapter, a detection scheme for oligonucleotides that utilize Oligo-Au NPs is evaluated with multiple oligonucleotide targets. This scheme takes advantage of the sharp melting transitions, intense optical properties, catalytic properties, enhanced binding properties, and the programmable assembly/disassembly of Oligo-Au NPs.",
keywords = "Biomolecule, Detection, DNA, Gold nanoparticle, Multiplexing, Oligonucleotide, RNA, Sensing",
author = "Lee, {Jae Seung}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC 2011. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2011",
doi = "10.1007/978-1-61779-052-2_2",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "17--31",
booktitle = "Methods in Molecular Biology",
}