Multiplexed detection of oligonucleotides with biobarcoded gold nanoparticle probes

Jae Seung Lee

    Research output: Chapter in Book/Report/Conference proceedingChapter

    4 Citations (Scopus)

    Abstract

    Applications in a variety of fields rely on the high-throughput ultrasensitive and multiplexed detection of oligonucleotides. However, the conventional microarray-based techniques that employ fluorescent dyes are hampered by several limitations; they require target amplification, fluorophore labeling, and complicated instrumentation, while the fluorophore-labeled species themselves exhibit slow binding kinetics, photo-bleaching effects, and overlapping spectral profiles. Among the emerging nanomaterials that are being used to solve these problems, oligonucleotide-gold nanoparticle conjugates (Oligo-Au NPs) have recently been highlighted due to their unique chemical and physical properties. In this chapter, a detection scheme for oligonucleotides that utilize Oligo-Au NPs is evaluated with multiple oligonucleotide targets. This scheme takes advantage of the sharp melting transitions, intense optical properties, catalytic properties, enhanced binding properties, and the programmable assembly/disassembly of Oligo-Au NPs.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages17-31
    Number of pages15
    DOIs
    Publication statusPublished - 2011

    Publication series

    NameMethods in Molecular Biology
    Volume726
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    Keywords

    • Biomolecule
    • Detection
    • DNA
    • Gold nanoparticle
    • Multiplexing
    • Oligonucleotide
    • RNA
    • Sensing

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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