Muricauda ochracea sp. Nov., isolated from a tidal flat in the republic of korea

A yellowish-brown-coloured bacterium, designated strain JGD-17^T, was isolated from a tidal flat of Janggu-do, Garorim bay, Taean-gun, Chungcheongbuk-do, Republic of Korea. Cells were Gram-stain-negative, aerobic, non-flagellated and long-rod-shaped. Growth was observed at 20–45 °C (optimum, 25–30 °C), at pH 6.0–10.0 (9.0) and with 1–5% (w/v) NaCl (1–3%). Results of 16S rRNA gene sequence analysis indicated that strain JGD-17^T was closely related to Muricauda nanhaiensis SM1704^T (96.1%), Muricauda olearia CL-SS4T (95.0%), Muricauda beolgyonensis BB-My12T (94.9%), Muricauda marina H19-56T (94.7%) and Muricauda indica 3PC125-7^T (94.5%). The ranges of values for the average nucleotide identity and digital DNA–DNA hybridiza-tion analyses with related strains were 71.3–74.1% and 16.9–18.2%. The genomic DNA G+C content was 41.1 mol%. Phylogenetic analysis using the neighbour-joining method showed that strain JGD-17^T formed a clade with Muricauda nanhaiensis SM1704^T, Muricauda lutaonensis CC-HSB-11^T, Muricauda lutea CSW06^T and Muricauda pacifica SM027^T. The major fatty acids were iso-C_15:0 (26.9%), iso-C_15:1 G (19.5%) and iso-C_17:0 3-OH (12.7%). The predominant respiratory quinone was menaquinone-6. The polar lipids were phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and two unidentified lipids. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain JGD-17^T represents a novel species within the genus Muricauda, for which the name Muricauda ochracea sp. nov. is proposed. The type strain is JGD-17^T (=KCTC 72732^T=KACC 21486^T=JCM 33817^T).