We examined the in vitro mutagenic and antimutagenic effects of hexane-extracted hemp (Cannabis sativa L. subsp. sativa var. sativa) seed oil (HO) with and without S9-mediated metabolic activation, using the TA98 and TA100 Salmonella Typhimurium strains. The MTT assay revealed no cytotoxicity in HepG2 cells for HO quantities ≤400 g/mL. In the mutagenicity test, revertant colonies did not exceed spontaneous colonies in number. Colony numbers did not increase in either strain after HO treatment, with or without metabolic activation. HO showed no mutagenic effects and did not induce a mutation in either strain. In the antimutagenicity test, HO reduced the number of mutated colonies induced by 4NQO in both strains. The inhibition rates of HO (TA98, 21-91%; TA100, 21-85%) indicated a potent reduction in mutagenicity induced by 4NQO. HO showed no significant mutagenicity and may have antimutagenic effects, as assessed by Ames testing.
Bibliographical noteFunding Information:
This work was supported by National Natural Science Foundation of China Grants 31630083 and 31572641; National Key Research and Development Program of China Grants 2018YFD0900503 and 2018YFD0900505; Stem Cell and Translational Research; National Key Research and Development Program of China Grant 2016YFA0101001; the Open Fund of the Labora-tory for Marine Biology and Biotechnology; Qingdao National Laboratory for Marine Science and Technology, Qingdao, China, Grant OF2017NO02; the Open Funding Project of the State Key Laboratory of Bioreactor Engi-neering; and the Zhejiang Major Special Program of Breeding Grant 2016C02055-4. The authors declare that they have no conflicts of interest with the contents of this article.
- Ames test
- Hemp seed oil
ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology