Mutagenicity and oral toxicity studies of Terminalia chebula

Ji Hoon Kim, Yun Chang Koo, Chung Oui Hong, Sung Yong Yang, Woojin Jun, Kwang Won Lee

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


The fruit of Terminalia chebula Retz. (T. chebula), which is a member of the Combfreetaceae family, is used widely in Asian countries as a traditional folk medicine, and its extract has been reported to be an anticancer, antidiabetic and anticaries agent. In our previous study, chebulic acid isolated from T. chebula extract was confirmed to show antioxidant activity and protective action against endothelial cell dysfunction. In order to support the safety-in-use of the ethyl acetate (EtOAc)-soluble portion of a T. chebula ethanol extract containing 29.4% chebulic acid content, the prepared portion was tested in an in vitro mutagenicity assay, and a single- and 14-day repeated dose oral toxicity study. In the bacterial mutation assay, up to 5000 μg/mL concentration of the EtOAc-soluble portion, the numbers of colonies did not increase whether with or without metabolic activation. In the oral toxicity study, the single oral dose of the extract at 2000 mg/kg did not produce mortality or abnormal lesions in the internal organs of rats. The results of a 14-day orally repeated dose showed that the EtOAc-soluble portion of T. chebula ethanol extracts gave no adverse effects at dosages of 2000 mg/kg in rats in the study.

Original languageEnglish
Pages (from-to)39-47
Number of pages9
JournalPhytotherapy Research
Issue number1
Publication statusPublished - 2012 Jan


  • Terminalia chebula
  • bacterial reverse mutation assay
  • chebulic acid
  • genotoxicity
  • oral toxicity

ASJC Scopus subject areas

  • Pharmacology


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