Mutational Analysis of Cucumber Mosaic Virus Movement Protein Gene

Jin Sam You, Hyung Suk Baik, Kyung Hee Paek

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    The movement protein of cucumber mosaic virus (CMV) is required for cell-to-cell movement of viral RNA. The movement of viral RNA occurs through the plant intercellular connection, the plasmodesmata. The viral movement protein was known to be multifunctional. In this work, a series of deletion mutants of CMV movement protein gene were created to identify the functional domains. The mutated movement proteins were produced as inclusion body in E. coli, and purified and renatured. A polyclonal antibody was raised against the CMV-Kor strain (Korean isolate) movement protein expressed in E. coli. The ability of the truncated proteins to bind to ssRNA was assayed by UV cross-linking and gel retardation analyses. The results indicate that the domain between amino acids 118 and 160 of CMV movement protein is essential for ssRNA binding.

    Original languageEnglish
    Pages (from-to)82-85
    Number of pages4
    JournalJournal of Biochemistry and Molecular Biology
    Volume32
    Issue number1
    Publication statusPublished - 1999 Jan 31

    Keywords

    • CMV-Kor
    • Movement protein
    • ssRNA binding

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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