Mutations in ArgS Arginine-tRNA Synthetase Confer Additional Antibiotic Tolerance Protection to Extended-Spectrum-β-Lactamase-Producing Burkholderia thailandensis

Hyojeong Yi, Jongwook Park, Kwang Hwi Cho, Heenam Stanley Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Highly conserved PenI-type class A β-lactamase in pathogenic members of Burkholderia species can evolve to extended-spectrum β-lactamase (ESBL), which exhibits hydrolytic activity toward third-generation cephalosporins, while losing its activity toward the original penicillin substrates. We describe three single-amino-acid-substitution mutations in the ArgS arginine-tRNA synthetase that confer extra antibiotic tolerance protection to ESBL-producing Burkholderia thailandensis. This pathway can be exploited to evade antibiotic tolerance induction in developing therapeutic measures against Burkholderia species, targeting their essential aminoacyl-tRNA synthetases.

Original languageEnglish
Article numbere02252-19
JournalAntimicrobial Agents and Chemotherapy
Volume64
Issue number6
DOIs
Publication statusPublished - 2020 Jun

Bibliographical note

Funding Information:
This work was supported by grants NRF-2018R1A2B2006456, 2018M3A9F3055923, and 2015M3C9A4053393 from the National Research Foundation (NRF) of the Republic of Korea. Additional support was provided by grant 2016R1A6A3A11935950 for research fellows from the NRF of the Republic of Korea to H.Y.

Publisher Copyright:
Copyright © 2020 American Society for Microbiology. All Rights Reserved.

Keywords

  • Antibiotic tolerance
  • ArgS
  • Arginine-tRNA synthetase
  • Stringent response

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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