Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare

So Young Lee; Byoung Jun Kim; Hong Kim; Yu Seop Won; Che Ok Jeon; Joseph Jeong; Seon Ho Lee; Ji Hun Lim; Seung Heon Lee; Chang Ki Kim; Yoon Hoh Kook; Bum Joon Kim

doi:10.1099/ijsem.0.001158

Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare

So Young Lee, Byoung Jun Kim, Hong Kim, Yu Seop Won, Che Ok Jeon, Joseph Jeong, Seon Ho Lee, Ji Hun Lim, Seung Heon Lee, Chang Ki Kim, Yoon Hoh Kook, Bum Joon Kim

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium intracellulare ATCC 13950^T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. intracellulare ATCC 13950^T. DNA-DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraintracellulare. The type strain is MOTT64^T (=KCTC 29084^T =JCM 30622^T).

Original language	English
Article number	001158
Pages (from-to)	3132-3141
Number of pages	10
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	66
Issue number	8
DOIs	https://doi.org/10.1099/ijsem.0.001158
Publication status	Published - 2016 Aug
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation of Korea (NRF) grant (no. NRF-2014R1A1A2004008), Republic of Korea.

Publisher Copyright:
© 2016 IUMS.

Keywords

Multilocus sequence typing analysis (MLST)
Mycobacterium avium complex
Mycobacterium intracellulare INT 1 group
Mycobacterium paraintracellulare

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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10.1099/ijsem.0.001158

Cite this

Lee, S. Y., Kim, B. J., Kim, H., Won, Y. S., Jeon, C. O., Jeong, J., Lee, S. H., Lim, J. H., Lee, S. H., Kim, C. K., Kook, Y. H., & Kim, B. J. (2016). Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare. International Journal of Systematic and Evolutionary Microbiology, 66(8), 3132-3141. Article 001158. https://doi.org/10.1099/ijsem.0.001158

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title = "Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare",

abstract = "Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. intracellulare ATCC 13950T. DNA-DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraintracellulare. The type strain is MOTT64T (=KCTC 29084T =JCM 30622T).",

keywords = "Multilocus sequence typing analysis (MLST), Mycobacterium avium complex, Mycobacterium intracellulare INT 1 group, Mycobacterium paraintracellulare",

author = "Lee, {So Young} and Kim, {Byoung Jun} and Hong Kim and Won, {Yu Seop} and Jeon, {Che Ok} and Joseph Jeong and Lee, {Seon Ho} and Lim, {Ji Hun} and Lee, {Seung Heon} and Kim, {Chang Ki} and Kook, {Yoon Hoh} and Kim, {Bum Joon}",

note = "Funding Information: This work was supported by a National Research Foundation of Korea (NRF) grant (no. NRF-2014R1A1A2004008), Republic of Korea. Publisher Copyright: {\textcopyright} 2016 IUMS.",

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AU - Lee, So Young

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AU - Kim, Hong

AU - Won, Yu Seop

AU - Jeon, Che Ok

AU - Jeong, Joseph

AU - Lee, Seon Ho

AU - Lim, Ji Hun

AU - Lee, Seung Heon

AU - Kim, Chang Ki

AU - Kook, Yoon Hoh

AU - Kim, Bum Joon

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AB - Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. intracellulare ATCC 13950T. DNA-DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraintracellulare. The type strain is MOTT64T (=KCTC 29084T =JCM 30622T).

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KW - Mycobacterium intracellulare INT 1 group

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Mycobacterium paraintracellulare sp. Nov., for the genotype INT-1 of Mycobacterium intracellulare

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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