Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. intracellulare ATCC 13950T. DNA-DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraintracellulare. The type strain is MOTT64T (=KCTC 29084T =JCM 30622T).
|Number of pages||10|
|Journal||International Journal of Systematic and Evolutionary Microbiology|
|Publication status||Published - 2016 Aug|
Bibliographical noteFunding Information:
This work was supported by a National Research Foundation of Korea (NRF) grant (no. NRF-2014R1A1A2004008), Republic of Korea.
© 2016 IUMS.
- Multilocus sequence typing analysis (MLST)
- Mycobacterium avium complex
- Mycobacterium intracellulare INT 1 group
- Mycobacterium paraintracellulare
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics