Mycobacterium tuberculosis lpdC, Rv0462, induces dendritic cell maturation and Th1 polarization

Deok Rim Heo; Sung Jae Shin; Woo Sik Kim; Kyung Tae Noh; Jin Wook Park; Kwang Hee Son; Won Sun Park; Min Goo Lee; Daejin Kim; Yong Kyoo Shin; In Duk Jung; Yeong Min Park

doi:10.1016/j.bbrc.2011.07.013

Mycobacterium tuberculosis lpdC, Rv0462, induces dendritic cell maturation and Th1 polarization

Deok Rim Heo, Sung Jae Shin, Woo Sik Kim, Kyung Tae Noh, Jin Wook Park, Kwang Hee Son, Won Sun Park, Min Goo Lee, Daejin Kim, Yong Kyoo Shin, In Duk Jung, Yeong Min Park

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Mycobacterium tuberculosis, the etiological factor of pulmonary tuberculosis, causes significant morbidity and mortality worldwide. Activation of host immune responses for containment of mycobacterial infections involves participation of innate immune cells, such as dendritic cells (DCs). In this study, we demonstrated that the gene encoding lipoamide dehydrogenase C (lpdC) from M. tuberculosis, Rv0462, induce maturation and activation of DCs involved in the MAPKs signaling pathway. Moreover, Rv0462-treated DCs activated naïve T cells, polarized CD4⁺ and CD8⁺ T cells to secrete IFN-γ in syngeneic mixed lymphocyte reactions, which would be expected to contribute to Th1 polarization of the immune response. Our results suggest that Rv0462 can contribute to the innate and adaptive immune responses during tuberculosis infection, and thus modulate the clinical course of tuberculosis.

Original language	English
Pages (from-to)	642-647
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	411
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2011.07.013
Publication status	Published - 2011 Aug 5
Externally published	Yes

Keywords

Dendritic cells
MAPKs
Mycobacterium tuberculosis
Rv0462
Th1 polarization

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2011.07.013

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title = "Mycobacterium tuberculosis lpdC, Rv0462, induces dendritic cell maturation and Th1 polarization",

abstract = "Mycobacterium tuberculosis, the etiological factor of pulmonary tuberculosis, causes significant morbidity and mortality worldwide. Activation of host immune responses for containment of mycobacterial infections involves participation of innate immune cells, such as dendritic cells (DCs). In this study, we demonstrated that the gene encoding lipoamide dehydrogenase C (lpdC) from M. tuberculosis, Rv0462, induce maturation and activation of DCs involved in the MAPKs signaling pathway. Moreover, Rv0462-treated DCs activated na{\"i}ve T cells, polarized CD4+ and CD8+ T cells to secrete IFN-γ in syngeneic mixed lymphocyte reactions, which would be expected to contribute to Th1 polarization of the immune response. Our results suggest that Rv0462 can contribute to the innate and adaptive immune responses during tuberculosis infection, and thus modulate the clinical course of tuberculosis.",

keywords = "Dendritic cells, MAPKs, Mycobacterium tuberculosis, Rv0462, Th1 polarization",

author = "Heo, {Deok Rim} and Shin, {Sung Jae} and Kim, {Woo Sik} and Noh, {Kyung Tae} and Park, {Jin Wook} and Son, {Kwang Hee} and Park, {Won Sun} and Lee, {Min Goo} and Daejin Kim and Shin, {Yong Kyoo} and Jung, {In Duk} and Park, {Yeong Min}",

note = "Funding Information: This study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry for Health Welfare & Family Affairs, Republic of Korea ( A091047-1012-0000300 ) and was financially supported by Pusan National University in program (Post-Doc. 2010).",

year = "2011",

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doi = "10.1016/j.bbrc.2011.07.013",

language = "English",

volume = "411",

pages = "642--647",

journal = "Biochemical and Biophysical Research Communications",

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AU - Heo, Deok Rim

AU - Shin, Sung Jae

AU - Kim, Woo Sik

AU - Noh, Kyung Tae

AU - Park, Jin Wook

AU - Son, Kwang Hee

AU - Park, Won Sun

AU - Lee, Min Goo

AU - Kim, Daejin

AU - Shin, Yong Kyoo

AU - Jung, In Duk

AU - Park, Yeong Min

N1 - Funding Information: This study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry for Health Welfare & Family Affairs, Republic of Korea ( A091047-1012-0000300 ) and was financially supported by Pusan National University in program (Post-Doc. 2010).

PY - 2011/8/5

Y1 - 2011/8/5

N2 - Mycobacterium tuberculosis, the etiological factor of pulmonary tuberculosis, causes significant morbidity and mortality worldwide. Activation of host immune responses for containment of mycobacterial infections involves participation of innate immune cells, such as dendritic cells (DCs). In this study, we demonstrated that the gene encoding lipoamide dehydrogenase C (lpdC) from M. tuberculosis, Rv0462, induce maturation and activation of DCs involved in the MAPKs signaling pathway. Moreover, Rv0462-treated DCs activated naïve T cells, polarized CD4+ and CD8+ T cells to secrete IFN-γ in syngeneic mixed lymphocyte reactions, which would be expected to contribute to Th1 polarization of the immune response. Our results suggest that Rv0462 can contribute to the innate and adaptive immune responses during tuberculosis infection, and thus modulate the clinical course of tuberculosis.

AB - Mycobacterium tuberculosis, the etiological factor of pulmonary tuberculosis, causes significant morbidity and mortality worldwide. Activation of host immune responses for containment of mycobacterial infections involves participation of innate immune cells, such as dendritic cells (DCs). In this study, we demonstrated that the gene encoding lipoamide dehydrogenase C (lpdC) from M. tuberculosis, Rv0462, induce maturation and activation of DCs involved in the MAPKs signaling pathway. Moreover, Rv0462-treated DCs activated naïve T cells, polarized CD4+ and CD8+ T cells to secrete IFN-γ in syngeneic mixed lymphocyte reactions, which would be expected to contribute to Th1 polarization of the immune response. Our results suggest that Rv0462 can contribute to the innate and adaptive immune responses during tuberculosis infection, and thus modulate the clinical course of tuberculosis.

KW - Dendritic cells

KW - MAPKs

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KW - Th1 polarization

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Mycobacterium tuberculosis lpdC, Rv0462, induces dendritic cell maturation and Th1 polarization

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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