N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

Y. H. Noh; H. S. Cho; D. H. Kim; O. H. Kim; J. Park; S. A. Lee; H. S. Yang; D. S. Sohn; W. Kim; D. Kim; Y. H. Chung; K. Y. Kim; S. S. Kim; W. B. Lee

doi:10.1134/S0026893312040085

N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

Y. H. Noh, H. S. Cho, D. H. Kim, O. H. Kim, J. Park, S. A. Lee, H. S. Yang, D. S. Sohn, W. Kim, D. Kim, Y. H. Chung, K. Y. Kim, S. S. Kim, W. B. Lee

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.

Original language	English
Pages (from-to)	664-669
Number of pages	6
Journal	Molecular Biology
Volume	46
Issue number	5
DOIs	https://doi.org/10.1134/S0026893312040085
Publication status	Published - 2012
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by the Chung Ang Uni versity Research Grants in 2010.

Keywords

Akt
N-acetylcysteine
N-cadherin
P19 embryonic stem cell
differentiation

ASJC Scopus subject areas

Biophysics
Structural Biology

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10.1134/S0026893312040085

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@article{999ce44f34924517b183f720e5dfef77,

title = "N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation",

abstract = "We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.",

keywords = "Akt, N-acetylcysteine, N-cadherin, P19 embryonic stem cell, differentiation",

author = "Noh, {Y. H.} and Cho, {H. S.} and Kim, {D. H.} and Kim, {O. H.} and J. Park and Lee, {S. A.} and Yang, {H. S.} and Sohn, {D. S.} and W. Kim and D. Kim and Chung, {Y. H.} and Kim, {K. Y.} and Kim, {S. S.} and Lee, {W. B.}",

note = "Funding Information: This study was supported by the Chung Ang Uni versity Research Grants in 2010.",

year = "2012",

doi = "10.1134/S0026893312040085",

language = "English",

volume = "46",

pages = "664--669",

journal = "Molecular Biology",

issn = "0026-8933",

publisher = "Pleiades Publishing",

number = "5",

}

TY - JOUR

T1 - N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

AU - Noh, Y. H.

AU - Cho, H. S.

AU - Kim, D. H.

AU - Kim, O. H.

AU - Park, J.

AU - Lee, S. A.

AU - Yang, H. S.

AU - Sohn, D. S.

AU - Kim, W.

AU - Kim, D.

AU - Chung, Y. H.

AU - Kim, K. Y.

AU - Kim, S. S.

AU - Lee, W. B.

N1 - Funding Information: This study was supported by the Chung Ang Uni versity Research Grants in 2010.

PY - 2012

Y1 - 2012

N2 - We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.

AB - We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.

KW - Akt

KW - N-acetylcysteine

KW - N-cadherin

KW - P19 embryonic stem cell

KW - differentiation

UR - http://www.scopus.com/inward/record.url?scp=84867308346&partnerID=8YFLogxK

U2 - 10.1134/S0026893312040085

DO - 10.1134/S0026893312040085

M3 - Article

AN - SCOPUS:84867308346

SN - 0026-8933

VL - 46

SP - 664

EP - 669

JO - Molecular Biology

JF - Molecular Biology

IS - 5

ER -

N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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