N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

Y. H. Noh; H. S. Cho; D. H. Kim; O. H. Kim; J. Park; S. A. Lee; H. S. Yang; D. S. Sohn; W. Kim; D. Kim; Y. H. Chung; K. Y. Kim; S. S. Kim; W. B. Lee

doi:10.1134/S0026893312040085

N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

Y. H. Noh^*, H. S. Cho, D. H. Kim, O. H. Kim, J. Park, S. A. Lee, H. S. Yang, D. S. Sohn, W. Kim, D. Kim, Y. H. Chung, K. Y. Kim, S. S. Kim, W. B. Lee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.

Original language	English
Pages (from-to)	664-669
Number of pages	6
Journal	Molecular Biology
Volume	46
Issue number	5
DOIs	https://doi.org/10.1134/S0026893312040085
Publication status	Published - 2012
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by the Chung Ang Uni versity Research Grants in 2010.

Keywords

Akt
N-acetylcysteine
N-cadherin
P19 embryonic stem cell
differentiation

ASJC Scopus subject areas

Biophysics
Structural Biology

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10.1134/S0026893312040085

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@article{999ce44f34924517b183f720e5dfef77,

title = "N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation",

abstract = "We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.",

keywords = "Akt, N-acetylcysteine, N-cadherin, P19 embryonic stem cell, differentiation",

author = "Noh, \{Y. H.\} and Cho, \{H. S.\} and Kim, \{D. H.\} and Kim, \{O. H.\} and J. Park and Lee, \{S. A.\} and Yang, \{H. S.\} and Sohn, \{D. S.\} and W. Kim and D. Kim and Chung, \{Y. H.\} and Kim, \{K. Y.\} and Kim, \{S. S.\} and Lee, \{W. B.\}",

note = "Funding Information: This study was supported by the Chung Ang Uni versity Research Grants in 2010.",

year = "2012",

doi = "10.1134/S0026893312040085",

language = "English",

volume = "46",

pages = "664--669",

journal = "Molecular Biology",

issn = "0026-8933",

publisher = "Pleiades Publishing",

number = "5",

}

TY - JOUR

T1 - N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

AU - Noh, Y. H.

AU - Cho, H. S.

AU - Kim, D. H.

AU - Kim, O. H.

AU - Park, J.

AU - Lee, S. A.

AU - Yang, H. S.

AU - Sohn, D. S.

AU - Kim, W.

AU - Kim, D.

AU - Chung, Y. H.

AU - Kim, K. Y.

AU - Kim, S. S.

AU - Lee, W. B.

N1 - Funding Information: This study was supported by the Chung Ang Uni versity Research Grants in 2010.

PY - 2012

Y1 - 2012

N2 - We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.

AB - We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.

KW - Akt

KW - N-acetylcysteine

KW - N-cadherin

KW - P19 embryonic stem cell

KW - differentiation

UR - https://www.scopus.com/pages/publications/84867308346

U2 - 10.1134/S0026893312040085

DO - 10.1134/S0026893312040085

M3 - Article

AN - SCOPUS:84867308346

SN - 0026-8933

VL - 46

SP - 664

EP - 669

JO - Molecular Biology

JF - Molecular Biology

IS - 5

ER -

N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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