Nanoelectrical characterization of individual exosomes secreted by Aβ42-ingested cells using electrostatic force microscopy

Yeseong Choi, Su Mi Kim, Youhee Heo, Gyudo Lee, Ji Yoon Kang, Dae Sung Yoon

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Quantifying the physical properties of individual exosomes containing amyloid-β42 (Aβ42) is crucial for a better understanding of an underpinning mechanism of Alzheimer’s disease expression which is associated with the Aβ42 transfer. Because of the lack of proper tools, however, there have been very few studies on how the amount of Aβ42 affects the physical properties of exosomes. To answer the question, we investigated the physical properties of exosomes secreted by neuroblastoma by probing individual exosomes using electrostatic force microscopy. Interestingly, we observed that when the higher concentration of Aβ42 oligomers was fed to cells, the higher surface charge of the exosomes appeared. This result indicates that the exosomes contain more Aβ42 with the increase in Aβ42 concentration in cell media, implying that they serve as transport vesicles for Aβ42. Our approach could help to better understand how the neuronal exosomes are related to the propagation of neurodegenerative diseases and to seek how to make an early diagnosis of those diseases.

Original languageEnglish
Article number025705
Issue number2
Publication statusPublished - 2021 Jan 8

Bibliographical note

Publisher Copyright:
© 2020 IOP Publishing Ltd Printed in the UK


  • Alzheimer’s disease
  • Amyloid β
  • Atomic force microscopy
  • Electrostatic force microscopy
  • Exosome

ASJC Scopus subject areas

  • Bioengineering
  • General Chemistry
  • General Materials Science
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering


Dive into the research topics of 'Nanoelectrical characterization of individual exosomes secreted by Aβ42-ingested cells using electrostatic force microscopy'. Together they form a unique fingerprint.

Cite this