Nanoliposomal Ratiometric Fluorescent Probe toward ONOO-Flux

Jie Zhang, Jianfei Kan, Yanyan Sun, Miae Won, Ji Hyeon Kim, Weifen Zhang, Jin Zhou, Zhaosheng Qian, Jong Seung Kim

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Peroxynitrite (ONOO-), a powerful biological oxidant, is produced in the mitochondria and reacts with many biomolecular targets under various pathological conditions, leading to a range of disease states. In this work, we developed a nanoliposome-encapsulated ratiometrically fluorescent probe (NRF) based on a hemicyanine structure Cy-O obtained by facile synthesis. Upon reaction with ONOO-, the oxidation and hydrolysis of a π-conjugation system within the nanoliposome triggers a ratiometrically fluorescent response and a large-scale emission shift (238 nm), which provides a specific and sensitive means for the ONOO- detection. Moreover, we have performed DFT calculation at the 6-31+G(d,p) level using a suite of Gaussian 09 programs to obtain insights into the chemical structure optical properties of Cy-O. In addition, the practical applications of the nanoprobe to image exogenous and endogenous ONOO- were achieved further in live cells and animals triumphantly.

Original languageEnglish
Pages (from-to)2080-2088
Number of pages9
JournalACS Applied Bio Materials
Volume4
Issue number3
DOIs
Publication statusPublished - 2021 Mar 15

Bibliographical note

Funding Information:
We gratefully acknowledge financial support from the NSF of China (21705120 and 81973671), the Natural Science Foundation of Shandong Province, China (ZR2017LB016), the Project of Shandong Province Higher Educational Science and Technology Program (J17KB074), the Project of Shandong Province Higher Educational Outstanding Youth Innovation Team (2019KJM008), Chinese Medicine Science and Technology Development Project of Shandong Province (2017-208), Open Research Fund of Beijing National Laboratory for Molecular Sciences (BNLMS201812), National Research Foundation of Korea (CRI projects 2018R1A3B1052702 and 2019M3E5D1A01068998, J.S.K.), and Shandong Province Government-Sponsored Overseas Study Project and the experimental support from Laboratory of Applied Pharmacology of Weifang Medical University.

Publisher Copyright:
© 2021 American Chemical Society. All rights reserved.

Keywords

  • fluorescent probe
  • imaging
  • nanoliposome
  • peroxynitrite
  • ratiometric

ASJC Scopus subject areas

  • General Chemistry
  • Biomaterials
  • Biomedical Engineering
  • Biochemistry, medical

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