Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase

Kyo Seon Hwang; Sang Myung Lee; Kilho Eom; Jeong Hoon Lee; Yoon Sik Lee; Jung Ho Park; Dae Sung Yoon; Tae Song Kim

doi:10.1016/j.bios.2007.05.006

Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase

Kyo Seon Hwang
, Sang Myung Lee
, Kilho Eom
, Jeong Hoon Lee
, Yoon Sik Lee
, Jung Ho Park
, Dae Sung Yoon^*
, Tae Song Kim

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

We report the nanomechanical microcantilevers operated in vibration modes (oscillation) with use of RNA aptamers as receptor molecules for label-free detection of hepatitis C virus (HCV) helicase. The nanomechanical detection principle is that the ligand-receptor binding on the microcantilever surface induces the dynamic response change of microcantilevers. We implemented the label-free detection of HCV helicase in the low concentration as much as 100 pg/ml from measuring the dynamic response change of microcantilevers. Moreover, from the recent studies showing that the ligand-receptor binding generates the surface stress on the microcantilever, we estimate the surface stress, on the oscillating microcantilevers, induced by ligand-receptor binding, i.e. binding between HCV helicase and RNA aptamer. In this article, it is suggested that the oscillating microcantilevers with use of RNA aptamers as receptor molecules may enable one to implement the sensitive label-free detection of very small amount of small-scale proteins.

Original language	English
Pages (from-to)	459-465
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	23
Issue number	4
DOIs	https://doi.org/10.1016/j.bios.2007.05.006
Publication status	Published - 2007 Nov 30

Bibliographical note

Funding Information:
Three authors (K.S.H., S.M.L. and K.E.) made equal contribution to this work. We thank Prof. Byung-Gee Kim and Dr. Suhyung Cho for preparation of RNA aptamers and HCV helicase. The authors are very grateful for the financial support by Intelligent Microsystem Center sponsored by Korea Ministry of Science and Technology as a part of the 21st century's Frontier R&D Projects (grant MS-01-133-01) and National Core Research Center for Nanomedical Technology sponsored by KOSEF (grant R15-2004-024-00000-0).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

HCV helicase
Microcantilever
Nanomechanics
RNA aptamer
Resonant frequency

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

Access to Document

10.1016/j.bios.2007.05.006

Cite this

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title = "Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase",

abstract = "We report the nanomechanical microcantilevers operated in vibration modes (oscillation) with use of RNA aptamers as receptor molecules for label-free detection of hepatitis C virus (HCV) helicase. The nanomechanical detection principle is that the ligand-receptor binding on the microcantilever surface induces the dynamic response change of microcantilevers. We implemented the label-free detection of HCV helicase in the low concentration as much as 100 pg/ml from measuring the dynamic response change of microcantilevers. Moreover, from the recent studies showing that the ligand-receptor binding generates the surface stress on the microcantilever, we estimate the surface stress, on the oscillating microcantilevers, induced by ligand-receptor binding, i.e. binding between HCV helicase and RNA aptamer. In this article, it is suggested that the oscillating microcantilevers with use of RNA aptamers as receptor molecules may enable one to implement the sensitive label-free detection of very small amount of small-scale proteins.",

keywords = "HCV helicase, Microcantilever, Nanomechanics, RNA aptamer, Resonant frequency",

author = "Hwang, \{Kyo Seon\} and Lee, \{Sang Myung\} and Kilho Eom and Lee, \{Jeong Hoon\} and Lee, \{Yoon Sik\} and Park, \{Jung Ho\} and Yoon, \{Dae Sung\} and Kim, \{Tae Song\}",

note = "Funding Information: Three authors (K.S.H., S.M.L. and K.E.) made equal contribution to this work. We thank Prof. Byung-Gee Kim and Dr. Suhyung Cho for preparation of RNA aptamers and HCV helicase. The authors are very grateful for the financial support by Intelligent Microsystem Center sponsored by Korea Ministry of Science and Technology as a part of the 21st century's Frontier R\&D Projects (grant MS-01-133-01) and National Core Research Center for Nanomedical Technology sponsored by KOSEF (grant R15-2004-024-00000-0).",

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journal = "Biosensors and Bioelectronics",

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AU - Hwang, Kyo Seon

AU - Lee, Sang Myung

AU - Eom, Kilho

AU - Lee, Jeong Hoon

AU - Lee, Yoon Sik

AU - Park, Jung Ho

AU - Yoon, Dae Sung

AU - Kim, Tae Song

N1 - Funding Information: Three authors (K.S.H., S.M.L. and K.E.) made equal contribution to this work. We thank Prof. Byung-Gee Kim and Dr. Suhyung Cho for preparation of RNA aptamers and HCV helicase. The authors are very grateful for the financial support by Intelligent Microsystem Center sponsored by Korea Ministry of Science and Technology as a part of the 21st century's Frontier R&D Projects (grant MS-01-133-01) and National Core Research Center for Nanomedical Technology sponsored by KOSEF (grant R15-2004-024-00000-0).

PY - 2007/11/30

Y1 - 2007/11/30

N2 - We report the nanomechanical microcantilevers operated in vibration modes (oscillation) with use of RNA aptamers as receptor molecules for label-free detection of hepatitis C virus (HCV) helicase. The nanomechanical detection principle is that the ligand-receptor binding on the microcantilever surface induces the dynamic response change of microcantilevers. We implemented the label-free detection of HCV helicase in the low concentration as much as 100 pg/ml from measuring the dynamic response change of microcantilevers. Moreover, from the recent studies showing that the ligand-receptor binding generates the surface stress on the microcantilever, we estimate the surface stress, on the oscillating microcantilevers, induced by ligand-receptor binding, i.e. binding between HCV helicase and RNA aptamer. In this article, it is suggested that the oscillating microcantilevers with use of RNA aptamers as receptor molecules may enable one to implement the sensitive label-free detection of very small amount of small-scale proteins.

AB - We report the nanomechanical microcantilevers operated in vibration modes (oscillation) with use of RNA aptamers as receptor molecules for label-free detection of hepatitis C virus (HCV) helicase. The nanomechanical detection principle is that the ligand-receptor binding on the microcantilever surface induces the dynamic response change of microcantilevers. We implemented the label-free detection of HCV helicase in the low concentration as much as 100 pg/ml from measuring the dynamic response change of microcantilevers. Moreover, from the recent studies showing that the ligand-receptor binding generates the surface stress on the microcantilever, we estimate the surface stress, on the oscillating microcantilevers, induced by ligand-receptor binding, i.e. binding between HCV helicase and RNA aptamer. In this article, it is suggested that the oscillating microcantilevers with use of RNA aptamers as receptor molecules may enable one to implement the sensitive label-free detection of very small amount of small-scale proteins.

KW - HCV helicase

KW - Microcantilever

KW - Nanomechanics

KW - RNA aptamer

KW - Resonant frequency

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Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase

Abstract

Bibliographical note

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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