Nanoscale enzyme reactors in mesoporous carbon for improved performance and lifetime of biosensors and biofuel cells

Ki Young Kwon, Jongkyu Youn, Jae Hyun Kim, Yongjin Park, Chulmin Jeon, Byoung Chan Kim, Yongchai Kwon, Xueyan Zhao, Ping Wang, Byoung In Sang, Jinwoo Lee, Hyun Gyu Park, Ho Nam Chang, Taeghwan Hyeon, Su Ha, Hee Tae Jung, Jungbae Kim

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)


Nanoscale enzyme reactors (NERs) of glucose oxidase in conductive mesoporous carbons were prepared in a two-step process of enzyme adsorption and follow-up enzyme crosslinking. MSU-F-C, a mesoprous carbon, has a bottleneck pore structure with mesocellular pores of 26. nm connected with window mesopores of 17. nm. This structure enables the ship-in-a-bottle mechanism of NERs, which effectively prevents the crosslinked enzymes in mesocellular pores from leaching through the smaller window mesopores. This NER approach not only stabilized the enzyme but also expedited electron transfer between the enzyme and the conductive MSU-F-C by maintaining a short distance between them. In a comparative study with GOx that was simply adsorbed without crosslinking, the NER approach was proven to be effective in improving the sensitivity of glucose biosensors and the power density of biofuel cells. The power density of biofuel cells could be further improved by manipulating several factors, such as by adding a mediator, changing the order of adsorption and crosslinking, and inserting a gold mesh as an electron collector.

Original languageEnglish
Pages (from-to)655-660
Number of pages6
JournalBiosensors and Bioelectronics
Issue number2
Publication statusPublished - 2010 Oct


  • Biofuel cells
  • Biosensors
  • Enzyme crosslinking
  • Mesoporous carbon
  • Nanoscale enzyme reactors

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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