Naturally-acquired immune response against Plasmodium vivax rhoptry-associated membrane antigen

Siriruk Changrob, Bo Wang, Jin Hee Han, Seong Kyun Lee, Myat Htut Nyunt, Chae Seung Lim, Takafumi Tsuboi, Patchanee Chootong, Eun Taek Han

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10 Citations (Scopus)


Rhoptry-associated membrane antigen (RAMA) is an abundant glycophosphatidylinositol (GPI)-anchored protein that is embedded within the lipid bilayer and is implicated in parasite invasion. Antibody responses against rhoptry proteins are produced by individuals living in a malaria-endemic area, suggesting the immunogenicity of Plasmodium vivax RAMA (PvRAMA) for induction of immune responses during P. vivax infection. To determine whether PvRAMA contributes to the acquisition of immunity to malaria and could be a rational candidate for a vaccine, the presence of memory T cells and the stability of the antibody response against PvRAMA were evaluated in P. vivax-exposed individuals. The immunogenicity of PvRAMA for the induction of T cell responses was evaluated by in vitro stimulation of peripheral blood mononuclear cells (PBMCs). High levels of interferon (IFN)-γ and interleukin (IL)-10 cytokines were detected in the culture supernatant of PBMCs, and the CD4+ T cells predominantly produced IL-10 cytokine. The levels of total anti-PvRAMA immunoglobulin G (IgG) antibody were significantly elevated, and these antibodies persisted over the 12 months of the study. Interestingly, IgG1, IgG2 and IgG3 were the major antibody subtypes in the response to PvRAMA. The frequency of IgG3 in specific to PvRAMA antigen maintained over 12 months. These data could explain the immunogenicity of PvRAMA antigen in induction of both cell-mediated and antibody-mediated immunity in natural P. vivax infection, in which IFN-γ helps antibody class switching toward the IgG1, IgG2 and IgG3 isotypes and IL-10 supports PvRAMA-specific antibody production.

Original languageEnglish
Article numbere0148723
JournalPloS one
Issue number2
Publication statusPublished - 2016 Feb

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. NRF-2014R1A2A1A11052079) and a grant from the Korea Health Technology R&D Project, the Ministry of Health & Welfare, Republic of Korea (A121180). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank all the staff at the Tha Sae and Malaria Clinic, Vector Borne Disease Control 11.4, Chumphon Province, Thailand for collection of the samples.

Publisher Copyright:
© 2016 Changrob et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ASJC Scopus subject areas

  • General


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