Two new cyanine-based fluorescent probes 1 and 2 have been developed. Probe 1 bears two cyanine units in a single molecule, and probe 2 contains a bis(trifluoromethyl)benzenethiol moiety. Both are non-fluorescent. The addition of intracellular glutathione (GSH) significantly enhanced the NIR fluorescence of the two probes. Both probes were used to image varying amounts of GSH in living cells. In tumor bearing mice, the in vivo fluorescence intensity of both probes was higher in tumors, where GSH is overexpressed, than in normal tissues. These results suggest that these new fluorogenic probes have potential for GSH-targeting diagnostic imaging.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (No. 2012R1A3A2048814 for J. Yoon). S. Kim acknowledges funding from the Korea Health Industry Development Institute (No. HI15C1540), the Innovative Medical Measurements Program from KRISS (KRISS-2017-GP2017-0020) and the Intramural Research Program of KIST. X. Chen acknowledges funding from the National Natural Science Foundation of China (21376117), the Jiangsu Natural Science Funds for Distinguished Young Scholars (BK20140043), and the Natural Science Foundation of Jiangsu Higher Education Institutions of China (14KJA150005). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer. The Korea Basic Science Institute (Seoul) is acknowledged for the LC/MS.
© 2018 The Royal Society of Chemistry.
ASJC Scopus subject areas
- Biomedical Engineering
- Materials Science(all)