Near-infrared fluorescent probes for the detection of glutathione and their application in the fluorescence imaging of living cells and tumor-bearing mice

Dayoung Lee; Keunsoo Jeong; Xiao Luo; Gayoung Kim; Youjun Yang; Xiaoqiang Chen; Sehoon Kim; Juyoung Yoon

doi:10.1039/c7tb01560g

Near-infrared fluorescent probes for the detection of glutathione and their application in the fluorescence imaging of living cells and tumor-bearing mice

Dayoung Lee, Keunsoo Jeong, Xiao Luo, Gayoung Kim, Youjun Yang, Xiaoqiang Chen, Sehoon Kim, Juyoung Yoon

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Two new cyanine-based fluorescent probes 1 and 2 have been developed. Probe 1 bears two cyanine units in a single molecule, and probe 2 contains a bis(trifluoromethyl)benzenethiol moiety. Both are non-fluorescent. The addition of intracellular glutathione (GSH) significantly enhanced the NIR fluorescence of the two probes. Both probes were used to image varying amounts of GSH in living cells. In tumor bearing mice, the in vivo fluorescence intensity of both probes was higher in tumors, where GSH is overexpressed, than in normal tissues. These results suggest that these new fluorogenic probes have potential for GSH-targeting diagnostic imaging.

Original language	English
Pages (from-to)	2541-2546
Number of pages	6
Journal	Journal of Materials Chemistry B
Volume	6
Issue number	17
DOIs	https://doi.org/10.1039/c7tb01560g
Publication status	Published - 2018

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (No. 2012R1A3A2048814 for J. Yoon). S. Kim acknowledges funding from the Korea Health Industry Development Institute (No. HI15C1540), the Innovative Medical Measurements Program from KRISS (KRISS-2017-GP2017-0020) and the Intramural Research Program of KIST. X. Chen acknowledges funding from the National Natural Science Foundation of China (21376117), the Jiangsu Natural Science Funds for Distinguished Young Scholars (BK20140043), and the Natural Science Foundation of Jiangsu Higher Education Institutions of China (14KJA150005). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer. The Korea Basic Science Institute (Seoul) is acknowledged for the LC/MS.

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

ASJC Scopus subject areas

General Chemistry
Biomedical Engineering
General Materials Science

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title = "Near-infrared fluorescent probes for the detection of glutathione and their application in the fluorescence imaging of living cells and tumor-bearing mice",

abstract = "Two new cyanine-based fluorescent probes 1 and 2 have been developed. Probe 1 bears two cyanine units in a single molecule, and probe 2 contains a bis(trifluoromethyl)benzenethiol moiety. Both are non-fluorescent. The addition of intracellular glutathione (GSH) significantly enhanced the NIR fluorescence of the two probes. Both probes were used to image varying amounts of GSH in living cells. In tumor bearing mice, the in vivo fluorescence intensity of both probes was higher in tumors, where GSH is overexpressed, than in normal tissues. These results suggest that these new fluorogenic probes have potential for GSH-targeting diagnostic imaging.",

author = "Dayoung Lee and Keunsoo Jeong and Xiao Luo and Gayoung Kim and Youjun Yang and Xiaoqiang Chen and Sehoon Kim and Juyoung Yoon",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (No. 2012R1A3A2048814 for J. Yoon). S. Kim acknowledges funding from the Korea Health Industry Development Institute (No. HI15C1540), the Innovative Medical Measurements Program from KRISS (KRISS-2017-GP2017-0020) and the Intramural Research Program of KIST. X. Chen acknowledges funding from the National Natural Science Foundation of China (21376117), the Jiangsu Natural Science Funds for Distinguished Young Scholars (BK20140043), and the Natural Science Foundation of Jiangsu Higher Education Institutions of China (14KJA150005). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer. The Korea Basic Science Institute (Seoul) is acknowledged for the LC/MS. Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",

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AU - Lee, Dayoung

AU - Jeong, Keunsoo

AU - Luo, Xiao

AU - Kim, Gayoung

AU - Yang, Youjun

AU - Chen, Xiaoqiang

AU - Kim, Sehoon

AU - Yoon, Juyoung

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (No. 2012R1A3A2048814 for J. Yoon). S. Kim acknowledges funding from the Korea Health Industry Development Institute (No. HI15C1540), the Innovative Medical Measurements Program from KRISS (KRISS-2017-GP2017-0020) and the Intramural Research Program of KIST. X. Chen acknowledges funding from the National Natural Science Foundation of China (21376117), the Jiangsu Natural Science Funds for Distinguished Young Scholars (BK20140043), and the Natural Science Foundation of Jiangsu Higher Education Institutions of China (14KJA150005). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer. The Korea Basic Science Institute (Seoul) is acknowledged for the LC/MS. Publisher Copyright: © 2018 The Royal Society of Chemistry.

PY - 2018

Y1 - 2018

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Near-infrared fluorescent probes for the detection of glutathione and their application in the fluorescence imaging of living cells and tumor-bearing mice

Abstract

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