Negative regulatory role of Annexin-A1 in 14-3-3η-mediated glucocorticoid receptor transcriptional activation

Ho Joong Sung; Yong Suk Ryang; Yoon Suk Kim; Sung Wuk Jang; Doe Sun Na; Jesang Ko

doi:10.1016/j.bbrc.2005.11.134

Negative regulatory role of Annexin-A1 in 14-3-3η-mediated glucocorticoid receptor transcriptional activation

Ho Joong Sung, Yong Suk Ryang, Yoon Suk Kim, Sung Wuk Jang, Doe Sun Na, Jesang Ko

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Annexin-A1 (ANX-1) is involved in glucocorticoid receptor (GR)-mediated signal transduction. However, the molecular mechanism by which ANX-1 plays a role in GR signaling is not fully understood. Recently, we reported that 14-3-3η inhibits degradation of GR, resulting in an increase in GR transcriptional activity. In this study, we have addressed the role of ANX-1 in 14-3-3η-induced enhancement of GR transactivation. ANX-1 abolished the increase in GR transcriptional activity due to 14-3-3η. ANX-1 had no effect on the protein level of GR. However, ANX-1 abrogated the inhibitory effect of 14-3-3η on GR degradation, which causes a decrease in GR stability in the nucleus. Our results indicate that ANX-1 functions as a negative regulator of GR transcriptional activation by inhibiting 14-3-3η-induced up-regulation of GR.

Original language	English
Pages (from-to)	1208-1211
Number of pages	4
Journal	Biochemical and biophysical research communications
Volume	339
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2005.11.134
Publication status	Published - 2006 Jan 27

Bibliographical note

Funding Information:
This work was supported by grant from Molecular and Cellular Bio-Discovery Research Program (M10401000004-04N0100-00410) from the Ministry of Science and Technology, South Korea.

Keywords

14-3-3η
Annexin-A1
Dexamethasone
Glucocorticoid receptor
Inflammation
Transcriptional activation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.11.134

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@article{01376ba7979342ad8bbccd9bcf871bc2,

title = "Negative regulatory role of Annexin-A1 in 14-3-3η-mediated glucocorticoid receptor transcriptional activation",

abstract = "Annexin-A1 (ANX-1) is involved in glucocorticoid receptor (GR)-mediated signal transduction. However, the molecular mechanism by which ANX-1 plays a role in GR signaling is not fully understood. Recently, we reported that 14-3-3η inhibits degradation of GR, resulting in an increase in GR transcriptional activity. In this study, we have addressed the role of ANX-1 in 14-3-3η-induced enhancement of GR transactivation. ANX-1 abolished the increase in GR transcriptional activity due to 14-3-3η. ANX-1 had no effect on the protein level of GR. However, ANX-1 abrogated the inhibitory effect of 14-3-3η on GR degradation, which causes a decrease in GR stability in the nucleus. Our results indicate that ANX-1 functions as a negative regulator of GR transcriptional activation by inhibiting 14-3-3η-induced up-regulation of GR.",

keywords = "14-3-3η, Annexin-A1, Dexamethasone, Glucocorticoid receptor, Inflammation, Transcriptional activation",

author = "Sung, {Ho Joong} and Ryang, {Yong Suk} and Kim, {Yoon Suk} and Jang, {Sung Wuk} and Na, {Doe Sun} and Jesang Ko",

note = "Funding Information: This work was supported by grant from Molecular and Cellular Bio-Discovery Research Program (M10401000004-04N0100-00410) from the Ministry of Science and Technology, South Korea.",

year = "2006",

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doi = "10.1016/j.bbrc.2005.11.134",

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AU - Sung, Ho Joong

AU - Ryang, Yong Suk

AU - Kim, Yoon Suk

AU - Jang, Sung Wuk

AU - Na, Doe Sun

AU - Ko, Jesang

N1 - Funding Information: This work was supported by grant from Molecular and Cellular Bio-Discovery Research Program (M10401000004-04N0100-00410) from the Ministry of Science and Technology, South Korea.

PY - 2006/1/27

Y1 - 2006/1/27

N2 - Annexin-A1 (ANX-1) is involved in glucocorticoid receptor (GR)-mediated signal transduction. However, the molecular mechanism by which ANX-1 plays a role in GR signaling is not fully understood. Recently, we reported that 14-3-3η inhibits degradation of GR, resulting in an increase in GR transcriptional activity. In this study, we have addressed the role of ANX-1 in 14-3-3η-induced enhancement of GR transactivation. ANX-1 abolished the increase in GR transcriptional activity due to 14-3-3η. ANX-1 had no effect on the protein level of GR. However, ANX-1 abrogated the inhibitory effect of 14-3-3η on GR degradation, which causes a decrease in GR stability in the nucleus. Our results indicate that ANX-1 functions as a negative regulator of GR transcriptional activation by inhibiting 14-3-3η-induced up-regulation of GR.

AB - Annexin-A1 (ANX-1) is involved in glucocorticoid receptor (GR)-mediated signal transduction. However, the molecular mechanism by which ANX-1 plays a role in GR signaling is not fully understood. Recently, we reported that 14-3-3η inhibits degradation of GR, resulting in an increase in GR transcriptional activity. In this study, we have addressed the role of ANX-1 in 14-3-3η-induced enhancement of GR transactivation. ANX-1 abolished the increase in GR transcriptional activity due to 14-3-3η. ANX-1 had no effect on the protein level of GR. However, ANX-1 abrogated the inhibitory effect of 14-3-3η on GR degradation, which causes a decrease in GR stability in the nucleus. Our results indicate that ANX-1 functions as a negative regulator of GR transcriptional activation by inhibiting 14-3-3η-induced up-regulation of GR.

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KW - Inflammation

KW - Transcriptional activation

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Negative regulatory role of Annexin-A1 in 14-3-3η-mediated glucocorticoid receptor transcriptional activation

Abstract

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