Neoagarooligosaccharides prevent septic shock by modulating A20-and cyclooxygenase-2-mediated interleukin-10 secretion in a septic-shock mouse model

Da Rae Kang; Gun Young Yoon; Joon Cho; Seung Jun Lee; Su Jin Lee; Hee Jo Park; Tae Heung Kang; Hee Dong Han; Won Sun Park; Young Kyung Yoon; Yeong Min Park; In Duk Jung

doi:10.1016/j.bbrc.2017.03.152

Neoagarooligosaccharides prevent septic shock by modulating A20-and cyclooxygenase-2-mediated interleukin-10 secretion in a septic-shock mouse model

Da Rae Kang, Gun Young Yoon, Joon Cho, Seung Jun Lee, Su Jin Lee, Hee Jo Park, Tae Heung Kang, Hee Dong Han, Won Sun Park, Young Kyung Yoon, Yeong Min Park, In Duk Jung

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Analysis of the signaling mechanism triggered by endotoxin-mediated toll-like receptor-4 activation using immune cell systems or rodent models may help identify potential agents for the prevention of Gram-negative bacteria infection. β-agarase cleaves the β-1,4-linkages of agar to produce neoagarooligosaccharides (NAOs), which have various physiological functions. The aim of this study was to investigate the efficacy of NAOs in preventing experimental sepsis caused by the administration of endotoxin or Gram-negative bacteria. Organ damage and neutrophil infiltration in an endotoxemia and septic-shock mouse model were suppressed by NAOs. Pro-inflammatory cytokine level was decreased, but IL-10 level was increased by NAO-treatment. Further induction by NAOs in the presence of endotoxin was associated with a significant induction of A20 and cyclooxygenase (COX)-2 expressions. Our data suggest that NAOs have a beneficial preventive effect in septic shock correlated with the enhancement of IL-10 via the induction of A20 and COX-2.

Original language	English
Pages (from-to)	998-1004
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	486
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2017.03.152
Publication status	Published - 2017 May 13
Externally published	Yes

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2013R1A4A1069575 , 2015R1A2A1A13001713 , 2015R1C1A1A02036329 , 2016R1D1A3B03934384 , and 2016R1A5A2012284 ).

Publisher Copyright:
© 2017 Elsevier Inc.

Keywords

A20
COX-2
IL-10
Neoagarooligosaccharides
Prevention
Sepsis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2017.03.152

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Kang, D. R., Yoon, G. Y., Cho, J., Lee, S. J., Lee, S. J., Park, H. J., Kang, T. H., Han, H. D., Park, W. S., Yoon, Y. K., Park, Y. M., & Jung, I. D. (2017). Neoagarooligosaccharides prevent septic shock by modulating A20-and cyclooxygenase-2-mediated interleukin-10 secretion in a septic-shock mouse model. Biochemical and biophysical research communications, 486(4), 998-1004. https://doi.org/10.1016/j.bbrc.2017.03.152

Kang, DR, Yoon, GY, Cho, J, Lee, SJ, Lee, SJ, Park, HJ, Kang, TH, Han, HD, Park, WS, Yoon, YK, Park, YM & Jung, ID 2017, 'Neoagarooligosaccharides prevent septic shock by modulating A20-and cyclooxygenase-2-mediated interleukin-10 secretion in a septic-shock mouse model', Biochemical and biophysical research communications, vol. 486, no. 4, pp. 998-1004. https://doi.org/10.1016/j.bbrc.2017.03.152

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abstract = "Analysis of the signaling mechanism triggered by endotoxin-mediated toll-like receptor-4 activation using immune cell systems or rodent models may help identify potential agents for the prevention of Gram-negative bacteria infection. β-agarase cleaves the β-1,4-linkages of agar to produce neoagarooligosaccharides (NAOs), which have various physiological functions. The aim of this study was to investigate the efficacy of NAOs in preventing experimental sepsis caused by the administration of endotoxin or Gram-negative bacteria. Organ damage and neutrophil infiltration in an endotoxemia and septic-shock mouse model were suppressed by NAOs. Pro-inflammatory cytokine level was decreased, but IL-10 level was increased by NAO-treatment. Further induction by NAOs in the presence of endotoxin was associated with a significant induction of A20 and cyclooxygenase (COX)-2 expressions. Our data suggest that NAOs have a beneficial preventive effect in septic shock correlated with the enhancement of IL-10 via the induction of A20 and COX-2.",

keywords = "A20, COX-2, IL-10, Neoagarooligosaccharides, Prevention, Sepsis",

author = "Kang, {Da Rae} and Yoon, {Gun Young} and Joon Cho and Lee, {Seung Jun} and Lee, {Su Jin} and Park, {Hee Jo} and Kang, {Tae Heung} and Han, {Hee Dong} and Park, {Won Sun} and Yoon, {Young Kyung} and Park, {Yeong Min} and Jung, {In Duk}",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2013R1A4A1069575 , 2015R1A2A1A13001713 , 2015R1C1A1A02036329 , 2016R1D1A3B03934384 , and 2016R1A5A2012284 ). Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

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AU - Yoon, Gun Young

AU - Cho, Joon

AU - Lee, Seung Jun

AU - Lee, Su Jin

AU - Park, Hee Jo

AU - Kang, Tae Heung

AU - Han, Hee Dong

AU - Park, Won Sun

AU - Yoon, Young Kyung

AU - Park, Yeong Min

AU - Jung, In Duk

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PY - 2017/5/13

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N2 - Analysis of the signaling mechanism triggered by endotoxin-mediated toll-like receptor-4 activation using immune cell systems or rodent models may help identify potential agents for the prevention of Gram-negative bacteria infection. β-agarase cleaves the β-1,4-linkages of agar to produce neoagarooligosaccharides (NAOs), which have various physiological functions. The aim of this study was to investigate the efficacy of NAOs in preventing experimental sepsis caused by the administration of endotoxin or Gram-negative bacteria. Organ damage and neutrophil infiltration in an endotoxemia and septic-shock mouse model were suppressed by NAOs. Pro-inflammatory cytokine level was decreased, but IL-10 level was increased by NAO-treatment. Further induction by NAOs in the presence of endotoxin was associated with a significant induction of A20 and cyclooxygenase (COX)-2 expressions. Our data suggest that NAOs have a beneficial preventive effect in septic shock correlated with the enhancement of IL-10 via the induction of A20 and COX-2.

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Neoagarooligosaccharides prevent septic shock by modulating A20-and cyclooxygenase-2-mediated interleukin-10 secretion in a septic-shock mouse model

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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