We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.
|Number of pages||8|
|Journal||Methods in Cell Science|
|Publication status||Published - 2003|
Bibliographical noteFunding Information:
We thank Neil Copeland for providing materials for BAC recombination. Confocal microscopy was performed using equipment made available by the Vanderbilt University Medical Center Cell Imaging Core Resource, supported by NIH grants 1S10RR15682-1, CA68485 and DK20593. Funds for this work were provided by the Vanderbilt University Academic Venture Capital Fund, the Whitehall Foundation, Inc., National Multiple Sclerosis Society, Christopher Reeve Paralysis Foundation and the National Institutes of Health.
- Motor neuron
- Purkinje neuron
- Spinal cord
ASJC Scopus subject areas
- Cell Biology