Neural cell fate analysis in zebrafish using olig2 BAC transgenics

Jimann Shin, Hae Chul Park, Jolanta M. Topczewska, David J. Madwsley, Bruce Appel

Research output: Contribution to journalArticlepeer-review

229 Citations (Scopus)


We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.

Original languageEnglish
Pages (from-to)7-14
Number of pages8
JournalMethods in Cell Science
Issue number1-2
Publication statusPublished - 2003
Externally publishedYes

Bibliographical note

Funding Information:
We thank Neil Copeland for providing materials for BAC recombination. Confocal microscopy was performed using equipment made available by the Vanderbilt University Medical Center Cell Imaging Core Resource, supported by NIH grants 1S10RR15682-1, CA68485 and DK20593. Funds for this work were provided by the Vanderbilt University Academic Venture Capital Fund, the Whitehall Foundation, Inc., National Multiple Sclerosis Society, Christopher Reeve Paralysis Foundation and the National Institutes of Health.


  • Cerebellum
  • EGFP
  • Motor neuron
  • Olig2
  • Oligodendrocyte
  • Purkinje neuron
  • Spinal cord
  • Transgenic
  • Zebrafish

ASJC Scopus subject areas

  • Cell Biology


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