Abstract
We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.
Original language | English |
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Pages (from-to) | 7-14 |
Number of pages | 8 |
Journal | Methods in Cell Science |
Volume | 25 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 2003 |
Externally published | Yes |
Keywords
- Cerebellum
- EGFP
- Motor neuron
- Olig2
- Oligodendrocyte
- Purkinje neuron
- Spinal cord
- Transgenic
- Zebrafish
ASJC Scopus subject areas
- Cell Biology