Neural cell fate analysis in zebrafish using olig2 BAC transgenics

Jimann Shin; Hae Chul Park; Jolanta M. Topczewska; David J. Madwsley; Bruce Appel

doi:10.1023/B:MICS.0000006847.09037.3a

Neural cell fate analysis in zebrafish using olig2 BAC transgenics

Jimann Shin, Hae Chul Park, Jolanta M. Topczewska, David J. Madwsley, Bruce Appel

Research output: Contribution to journal › Article › peer-review

225 Citations (Scopus)

Abstract

We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.

Original language	English
Pages (from-to)	7-14
Number of pages	8
Journal	Methods in Cell Science
Volume	25
Issue number	1-2
DOIs	https://doi.org/10.1023/B:MICS.0000006847.09037.3a
Publication status	Published - 2003
Externally published	Yes

Bibliographical note

Funding Information:
We thank Neil Copeland for providing materials for BAC recombination. Confocal microscopy was performed using equipment made available by the Vanderbilt University Medical Center Cell Imaging Core Resource, supported by NIH grants 1S10RR15682-1, CA68485 and DK20593. Funds for this work were provided by the Vanderbilt University Academic Venture Capital Fund, the Whitehall Foundation, Inc., National Multiple Sclerosis Society, Christopher Reeve Paralysis Foundation and the National Institutes of Health.

Keywords

Cerebellum
EGFP
Motor neuron
Olig2
Oligodendrocyte
Purkinje neuron
Spinal cord
Transgenic
Zebrafish

ASJC Scopus subject areas

Cell Biology

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10.1023/B:MICS.0000006847.09037.3a

Cite this

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title = "Neural cell fate analysis in zebrafish using olig2 BAC transgenics",

abstract = "We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.",

keywords = "Cerebellum, EGFP, Motor neuron, Olig2, Oligodendrocyte, Purkinje neuron, Spinal cord, Transgenic, Zebrafish",

author = "Jimann Shin and Park, {Hae Chul} and Topczewska, {Jolanta M.} and Madwsley, {David J.} and Bruce Appel",

note = "Funding Information: We thank Neil Copeland for providing materials for BAC recombination. Confocal microscopy was performed using equipment made available by the Vanderbilt University Medical Center Cell Imaging Core Resource, supported by NIH grants 1S10RR15682-1, CA68485 and DK20593. Funds for this work were provided by the Vanderbilt University Academic Venture Capital Fund, the Whitehall Foundation, Inc., National Multiple Sclerosis Society, Christopher Reeve Paralysis Foundation and the National Institutes of Health.",

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doi = "10.1023/B:MICS.0000006847.09037.3a",

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AU - Shin, Jimann

AU - Park, Hae Chul

AU - Topczewska, Jolanta M.

AU - Madwsley, David J.

AU - Appel, Bruce

N1 - Funding Information: We thank Neil Copeland for providing materials for BAC recombination. Confocal microscopy was performed using equipment made available by the Vanderbilt University Medical Center Cell Imaging Core Resource, supported by NIH grants 1S10RR15682-1, CA68485 and DK20593. Funds for this work were provided by the Vanderbilt University Academic Venture Capital Fund, the Whitehall Foundation, Inc., National Multiple Sclerosis Society, Christopher Reeve Paralysis Foundation and the National Institutes of Health.

PY - 2003

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N2 - We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.

AB - We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.

KW - Cerebellum

KW - EGFP

KW - Motor neuron

KW - Olig2

KW - Oligodendrocyte

KW - Purkinje neuron

KW - Spinal cord

KW - Transgenic

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Neural cell fate analysis in zebrafish using olig2 BAC transgenics

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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