Neuroglobin protects PC12 cells against β-amyloid-induced cell injury

Richard C. Li, Farzan Pouranfar, Seung Kwan Lee, Matthew W. Morris, Yang Wang, David Gozal

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)


Excessive accumulation of amyloid beta (Aβ) has been proposed as a pivotal event in the pathogenesis of Alzheimer's disease. Possible mechanisms underlying Aβ-induced neuronal cytotoxicity include excess production of reactive oxidative species (ROS) and apoptosis. Neuroglobin (Ngb), a newly discovered globin in vertebrates that exhibits neuroprotective functions, may have a potential role in scavenging ROS. To examine the potential protective role of Ngb in Aβ-induced cytotoxicity, PC12 cells were treated with Aβ (1-42 fragment) for 24 h. Aβ treatments increased ROS production in PC12 cells. Overexpression of Ngb but not Ngb mutant in the PC12 cells significantly attenuated Aβ-induced ROS production and lipids peroxidation. Furthermore, overexpression of Ngb also attenuated Aβ-induced mitochondrial dysfunction and apoptosis, and promoted cell survival in PC12 cells. Therefore, Ngb may act as an intracellular ROS scavenger, and such antioxidant properties may play a protective role against Aβ-induced cell injury.

Original languageEnglish
Pages (from-to)1815-1822
Number of pages8
JournalNeurobiology of Aging
Issue number12
Publication statusPublished - 2008 Dec
Externally publishedYes

Bibliographical note

Funding Information:
We thank Kenneth R. Brittian and Heather B. Clair for their expert technical assistance in cell culture and immunocytochemistry. This study was supported by National Institutes of Health grants SCOR 2P50-HL-60296 (Project 2) and RO1-HL-69932, The Children's Foundation Endowment for Sleep Research, and by the Commonwealth of Kentucky Challenge for Excellence Trust Fund.


  • Amyloid
  • Apoptosis
  • Neuroglobin
  • Oxidative stress

ASJC Scopus subject areas

  • General Neuroscience
  • Ageing
  • Developmental Biology
  • Clinical Neurology
  • Geriatrics and Gerontology


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