The separation and identification of siderophores produced by microorganisms is a time-consuming and an expensive procedure. We have developed a new and efficient method to identify siderophores using well-established Saccharomyces cerevisiae deletion mutants. The δfet3,arn strains fail to sustain growth, even when specific siderophores are supplied, and mutants are siderophore-specific: δfet3,arn2 for triacetylfusarinine C (TAFC), δfet3,arn1,sit1 for ferrichrome (FC), and δfet3,sit1 for ferrioxamine B (FOB). The culture broth of Fusarium graminearum was separated by HPLC, and each peak was subjected to a plate assay using S. cerevisiae mutants. We have found that each peak contained specific siderophores produced by F. graminearum, and these coincided with reference siderophores. This method is quite novel because nobody tried this method to identify the siderophores. Furthermore, this method will save time and cost in the identification of siderophores produced by microorganisms.
Bibliographical noteFunding Information:
This work was supported by Biogreen 21 of the Administration, Republic of Korea (No. 2005-
- F. graminearum
- S. cerevisiae
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