Abstract
Manganese (Mn2+)-enhanced MRI (MEMRI) is known to provide insight into functional and anatomical biology. However, this method, which uses Mn2+ as a MRI-detectable contrast agent, has drawbacks such as the toxicity to cells beyond a certain level of Mn2+. In this study, we attempt to determine a new method of ICV administration, the optimal concentration of administered Mn2+ and the optimal MEMRI acquisition time following administration. Male Sprague-Dawley rats were used in the following experimental sessions: (1) intracerebroventricular (ICV) cannula implantation in the region of the cisterna magna, (2) serial dilution of MnCl2 (20-80 mM), (3) ICV administration of MnCl2 through the cannula, and (4) T1-weighted MRI measurements. We confirmed that cannula implantation in the region of the cisterna magna was a new ICV injection method for the administration of a contrast agent. The optimal concentration for MEMRI was 20/50 mM/μl of MnCl2. The MEMRI data acquired at different time points indicate that most signal enhancement is maintained during 14-48 h after contrast agent injection, and 24 h was the optimal time to acquire images of the rat brain. The present study offers optimized parameters for contrast agent injection that would be a good basis for studies using MEMRI to research the rat brain.
Original language | English |
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Pages (from-to) | 157-164 |
Number of pages | 8 |
Journal | Experimental Animals |
Volume | 61 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2012 Apr 25 |
Keywords
- Cannula implantation
- Intracerebroventricular injection
- Manganese-enhanced MRI
- Mn
- Rat brain
ASJC Scopus subject areas
- Animal Science and Zoology
- General Biochemistry,Genetics and Molecular Biology
- General Veterinary