TY - JOUR
T1 - New role of human ribosomal protein S3
T2 - Regulation of cell cycle via phosphorylation by cyclin-dependent kinase 2
AU - Han, Se Hee
AU - Chung, Ji Hyung
AU - Kim, Joon
AU - Kim, Key Sun
AU - Han, Ye Sun
N1 - Funding Information:
This study was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant no. HI15C1540) and the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (no. NRF-2015R1C1A2A01053623).
Publisher Copyright:
© 2017, Spandidos Publications. All rights reserved.
PY - 2017/5
Y1 - 2017/5
N2 - Human ribosomal protein S3 (hRpS3) is a component of the 40S ribosomal subunit that associated in protein synthesis. hRpS3 has additional ribosomal functions such as DNA repair, transcription, metastasis, and apoptosis via interaction with numerous signaling molecules and has different modifications. Cyclin-dependent kinases (CDKs) are heterodimeric serine/threonine protein kinases that regulate cell cycle progression. Among its members, the Cdk1-cyclin B complex is known to control cell progression in the G2/M phase, while Cdk2-cyclin E/A complexes function in G1/S and S/G2 transition. In our previous study, we observed interaction between hRpS3 and Cdk1. The present study investigated the interaction between hRpS3 and Cdk2. Cdk2 phosphorylated hRps3 at amino acid residues S6 and T221 during the S-phase. Furthermore, hRpS3 knockdown delayed cell cycle progression by modulating the expression of cell cycle-related proteins, including cyclin B1 and cyclin E1. These findings suggest that hRpS3 is involved in Cdk2-mediated cell cycle regulation.
AB - Human ribosomal protein S3 (hRpS3) is a component of the 40S ribosomal subunit that associated in protein synthesis. hRpS3 has additional ribosomal functions such as DNA repair, transcription, metastasis, and apoptosis via interaction with numerous signaling molecules and has different modifications. Cyclin-dependent kinases (CDKs) are heterodimeric serine/threonine protein kinases that regulate cell cycle progression. Among its members, the Cdk1-cyclin B complex is known to control cell progression in the G2/M phase, while Cdk2-cyclin E/A complexes function in G1/S and S/G2 transition. In our previous study, we observed interaction between hRpS3 and Cdk1. The present study investigated the interaction between hRpS3 and Cdk2. Cdk2 phosphorylated hRps3 at amino acid residues S6 and T221 during the S-phase. Furthermore, hRpS3 knockdown delayed cell cycle progression by modulating the expression of cell cycle-related proteins, including cyclin B1 and cyclin E1. These findings suggest that hRpS3 is involved in Cdk2-mediated cell cycle regulation.
KW - Cell cycle regulation
KW - Cyclin-dependent kinase 2
KW - Human ribosomal protein S3
KW - In vitro kinase assay
KW - Protein-protein interaction
UR - http://www.scopus.com/inward/record.url?scp=85017416196&partnerID=8YFLogxK
U2 - 10.3892/ol.2017.5906
DO - 10.3892/ol.2017.5906
M3 - Article
AN - SCOPUS:85017416196
SN - 1792-1074
VL - 13
SP - 3681
EP - 3687
JO - Oncology Letters
JF - Oncology Letters
IS - 5
ER -
