TY - GEN
T1 - Next generation deformability cytometry
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
AU - Deng, Yanxiang
AU - Chung, Aram
PY - 2016/1/1
Y1 - 2016/1/1
N2 - We present next generation microfluidic deformability cytometry (NG-DC) capable of analyzing single-cell deformability close to real-time and in a fully automated manner at 2,000 cells/s. NG-DC is comprised of four processes: (1) Cell injection: cell suspensions are injected into a microchannel through a pressure controller, (2) Cell alignment and spacing: randomly incoming cells form a singlestream with controlled spacing by fluid inertia, (3) Cell stretch: cells deforms upon collision at a T-junction, and (4) Data processing: images of cell elongation motion are captured, analyzed, and displayed close to real-time. Through NG-DC, various cancer cell lines have been analyzed.
AB - We present next generation microfluidic deformability cytometry (NG-DC) capable of analyzing single-cell deformability close to real-time and in a fully automated manner at 2,000 cells/s. NG-DC is comprised of four processes: (1) Cell injection: cell suspensions are injected into a microchannel through a pressure controller, (2) Cell alignment and spacing: randomly incoming cells form a singlestream with controlled spacing by fluid inertia, (3) Cell stretch: cells deforms upon collision at a T-junction, and (4) Data processing: images of cell elongation motion are captured, analyzed, and displayed close to real-time. Through NG-DC, various cancer cell lines have been analyzed.
KW - Cell mechanophenotyping
KW - Inertial microfluidics
KW - Single-cell analysis
UR - http://www.scopus.com/inward/record.url?scp=85014234246&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014234246&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014234246
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 148
EP - 149
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
Y2 - 9 October 2016 through 13 October 2016
ER -
