Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping

Yanxiang Deng; Aram Chung

Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping

Yanxiang Deng
, Aram Chung^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

We present next generation microfluidic deformability cytometry (NG-DC) capable of analyzing single-cell deformability close to real-time and in a fully automated manner at 2,000 cells/s. NG-DC is comprised of four processes: (1) Cell injection: cell suspensions are injected into a microchannel through a pressure controller, (2) Cell alignment and spacing: randomly incoming cells form a singlestream with controlled spacing by fluid inertia, (3) Cell stretch: cells deforms upon collision at a T-junction, and (4) Data processing: images of cell elongation motion are captured, analyzed, and displayed close to real-time. Through NG-DC, various cancer cell lines have been analyzed.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	148-149
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016 Jan 1
Externally published	Yes
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13

Publication series

Name	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	16/10/9 → 16/10/13

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell mechanophenotyping
Inertial microfluidics
Single-cell analysis

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

Deng, Y., & Chung, A. (2016). Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 148-149). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.

Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping. / Deng, Yanxiang; Chung, Aram.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 148-149 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Deng, Y & Chung, A 2016, Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 148-149, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16/10/9.

Deng Y, Chung A. Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 148-149. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Deng, Yanxiang ; Chung, Aram. / Next generation deformability cytometry : Fully automated, high-throughput and near real-time cell mechanotyping. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 148-149 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

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title = "Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping",

abstract = "We present next generation microfluidic deformability cytometry (NG-DC) capable of analyzing single-cell deformability close to real-time and in a fully automated manner at 2,000 cells/s. NG-DC is comprised of four processes: (1) Cell injection: cell suspensions are injected into a microchannel through a pressure controller, (2) Cell alignment and spacing: randomly incoming cells form a singlestream with controlled spacing by fluid inertia, (3) Cell stretch: cells deforms upon collision at a T-junction, and (4) Data processing: images of cell elongation motion are captured, analyzed, and displayed close to real-time. Through NG-DC, various cancer cell lines have been analyzed.",

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author = "Yanxiang Deng and Aram Chung",

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language = "English",

series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

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N2 - We present next generation microfluidic deformability cytometry (NG-DC) capable of analyzing single-cell deformability close to real-time and in a fully automated manner at 2,000 cells/s. NG-DC is comprised of four processes: (1) Cell injection: cell suspensions are injected into a microchannel through a pressure controller, (2) Cell alignment and spacing: randomly incoming cells form a singlestream with controlled spacing by fluid inertia, (3) Cell stretch: cells deforms upon collision at a T-junction, and (4) Data processing: images of cell elongation motion are captured, analyzed, and displayed close to real-time. Through NG-DC, various cancer cell lines have been analyzed.

AB - We present next generation microfluidic deformability cytometry (NG-DC) capable of analyzing single-cell deformability close to real-time and in a fully automated manner at 2,000 cells/s. NG-DC is comprised of four processes: (1) Cell injection: cell suspensions are injected into a microchannel through a pressure controller, (2) Cell alignment and spacing: randomly incoming cells form a singlestream with controlled spacing by fluid inertia, (3) Cell stretch: cells deforms upon collision at a T-junction, and (4) Data processing: images of cell elongation motion are captured, analyzed, and displayed close to real-time. Through NG-DC, various cancer cell lines have been analyzed.

KW - Cell mechanophenotyping

KW - Inertial microfluidics

KW - Single-cell analysis

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M3 - Conference contribution

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BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

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Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping

Abstract

Publication series

Other

UN SDGs

Keywords

ASJC Scopus subject areas

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Cite this