Nicotine induces the expression of early growth response-1 in human skin dermal fibroblasts

Jae Eun Choi; Ji Na Kim; Sang Hoon Jeong; Sang Wook Son

doi:10.1111/j.1365-4632.2009.04210.x

Nicotine induces the expression of early growth response-1 in human skin dermal fibroblasts

Jae Eun Choi, Ji Na Kim, Sang Hoon Jeong, Sang Wook Son

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Background: Egr-1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response-1 (Egr-1) expression is unknown in HSDF. Methods: Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr-1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real-time reverse transcription-polymerase chain reaction. We also measured the promoter activity of Egr-1 in HSDF after nicotine exposure. Results: Early growth response-1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr-1 in cultured human fibroblasts. Conclusion: In this study, we demonstrate that Egr-1 expression is markedly induced in HSDF after exposure to nicotine.

Original language	English
Pages (from-to)	158-161
Number of pages	4
Journal	International Journal of Dermatology
Volume	49
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-4632.2009.04210.x
Publication status	Published - 2010 Feb

ASJC Scopus subject areas

Dermatology

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10.1111/j.1365-4632.2009.04210.x

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title = "Nicotine induces the expression of early growth response-1 in human skin dermal fibroblasts",

abstract = "Background: Egr-1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response-1 (Egr-1) expression is unknown in HSDF. Methods: Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr-1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real-time reverse transcription-polymerase chain reaction. We also measured the promoter activity of Egr-1 in HSDF after nicotine exposure. Results: Early growth response-1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr-1 in cultured human fibroblasts. Conclusion: In this study, we demonstrate that Egr-1 expression is markedly induced in HSDF after exposure to nicotine.",

author = "Choi, {Jae Eun} and Kim, {Ji Na} and Jeong, {Sang Hoon} and Son, {Sang Wook}",

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doi = "10.1111/j.1365-4632.2009.04210.x",

language = "English",

volume = "49",

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T1 - Nicotine induces the expression of early growth response-1 in human skin dermal fibroblasts

AU - Choi, Jae Eun

AU - Kim, Ji Na

AU - Jeong, Sang Hoon

AU - Son, Sang Wook

PY - 2010/2

Y1 - 2010/2

N2 - Background: Egr-1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response-1 (Egr-1) expression is unknown in HSDF. Methods: Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr-1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real-time reverse transcription-polymerase chain reaction. We also measured the promoter activity of Egr-1 in HSDF after nicotine exposure. Results: Early growth response-1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr-1 in cultured human fibroblasts. Conclusion: In this study, we demonstrate that Egr-1 expression is markedly induced in HSDF after exposure to nicotine.

AB - Background: Egr-1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response-1 (Egr-1) expression is unknown in HSDF. Methods: Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr-1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real-time reverse transcription-polymerase chain reaction. We also measured the promoter activity of Egr-1 in HSDF after nicotine exposure. Results: Early growth response-1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr-1 in cultured human fibroblasts. Conclusion: In this study, we demonstrate that Egr-1 expression is markedly induced in HSDF after exposure to nicotine.

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Nicotine induces the expression of early growth response-1 in human skin dermal fibroblasts

Abstract

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