TY - JOUR
T1 - Nicotine induces the expression of early growth response-1 in human skin dermal fibroblasts
AU - Choi, Jae Eun
AU - Kim, Ji Na
AU - Jeong, Sang Hoon
AU - Son, Sang Wook
PY - 2010/2
Y1 - 2010/2
N2 - Background: Egr-1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response-1 (Egr-1) expression is unknown in HSDF. Methods: Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr-1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real-time reverse transcription-polymerase chain reaction. We also measured the promoter activity of Egr-1 in HSDF after nicotine exposure. Results: Early growth response-1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr-1 in cultured human fibroblasts. Conclusion: In this study, we demonstrate that Egr-1 expression is markedly induced in HSDF after exposure to nicotine.
AB - Background: Egr-1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response-1 (Egr-1) expression is unknown in HSDF. Methods: Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr-1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real-time reverse transcription-polymerase chain reaction. We also measured the promoter activity of Egr-1 in HSDF after nicotine exposure. Results: Early growth response-1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr-1 in cultured human fibroblasts. Conclusion: In this study, we demonstrate that Egr-1 expression is markedly induced in HSDF after exposure to nicotine.
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U2 - 10.1111/j.1365-4632.2009.04210.x
DO - 10.1111/j.1365-4632.2009.04210.x
M3 - Article
C2 - 20465638
AN - SCOPUS:74949097183
SN - 0011-9059
VL - 49
SP - 158
EP - 161
JO - International Journal of Dermatology
JF - International Journal of Dermatology
IS - 2
ER -