Nicotine induces the expression of early growth response-1 in human skin dermal fibroblasts

Jae Eun Choi, Ji Na Kim, Sang Hoon Jeong, Sang Wook Son

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Background: Egr-1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response-1 (Egr-1) expression is unknown in HSDF. Methods: Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr-1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real-time reverse transcription-polymerase chain reaction. We also measured the promoter activity of Egr-1 in HSDF after nicotine exposure. Results: Early growth response-1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr-1 in cultured human fibroblasts. Conclusion: In this study, we demonstrate that Egr-1 expression is markedly induced in HSDF after exposure to nicotine.

    Original languageEnglish
    Pages (from-to)158-161
    Number of pages4
    JournalInternational Journal of Dermatology
    Volume49
    Issue number2
    DOIs
    Publication statusPublished - 2010 Feb

    ASJC Scopus subject areas

    • Dermatology

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