Nitrifying Bacterial Community Structure of a Full-Scale Integrated Fixed-Film Activated Sludge Process as Investigated by Pyrosequencing

Taek Seung Kim, Han Shin Kim, Soondong Kwon, Hee Deung Park

    Research output: Contribution to journalArticlepeer-review

    26 Citations (Scopus)

    Abstract

    Nitrifying bacterial community structures of suspended and attached biomasses in a full-scale integrated fixed-film activated sludge process were investigated by analyzing 16S rRNA gene sequences obtained from pyrosequencing. The suspended biomass had a higher number of ammonia-oxidizing bacterial sequences (0.8% of total sequences) than the attached biomass (0.07%), although most of the sequences were within the Nitrosomonas oligotropha lineage in both biomasses. Nitrospira-like nitrite-oxidizing bacterial sequences were retrieved in the suspended biomass (0.06%), not in the attached biomass, whereas the existence of Nitrobacter-like sequences was not evident. The suspended biomass had higher nitrification activity (1.13 mg N/TSS/h) than the attached biomass (0.07 mg N/TSS/h). Overall, the results made it possible to conclude the importance of the suspended biomass, rather than the attached biomass, in nitrification in the wastewater treatment process studied.

    Original languageEnglish
    Pages (from-to)293-298
    Number of pages6
    JournalJournal of microbiology and biotechnology
    Volume21
    Issue number3
    DOIs
    Publication statusPublished - 2011 Mar

    Keywords

    • AOB
    • Activated sludge
    • IFAS
    • NOB
    • Pyrosequencing

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

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