NMDA receptor-mediated calcium influx plays an essential role in myoblast fusion

Kun Ho Lee; Jae Yong Park; Kyungjin Kim

doi:10.1016/j.febslet.2004.10.076

NMDA receptor-mediated calcium influx plays an essential role in myoblast fusion

Kun Ho Lee, Jae Yong Park, Kyungjin Kim

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Ca ²⁺ influx is known to be prerequisite for myoblast fusion during skeletal muscle differentiation. Here, we show that the N-methyl-D-aspartate (NMDA) receptor is involved in the Ca ²⁺ influx of C2C12 myoblasts. NMDA receptor (NR) 1 and NR2D were expressed in the myoblasts during muscle differentiation. Using Ca ²⁺ imaging analysis, Ca ²⁺ influx through NRs was directly measured at a single-cell level. l-Glutamate increased myoblast fusion as well as intracellular Ca ²⁺ levels, and both effects were completely blocked by MK801, a selective antagonist of NRs. Furthermore, treatment with the Ca ²⁺ ionophore A23187 recovered MK801-mediated inhibition of myoblast fusion. These results suggest that the NRs may play an important role in myoblast fusion by mediating Ca ²⁺ influx.

Original language	English
Pages (from-to)	47-52
Number of pages	6
Journal	FEBS Letters
Volume	578
Issue number	1-2
DOIs	https://doi.org/10.1016/j.febslet.2004.10.076
Publication status	Published - 2004 Dec 3
Externally published	Yes

Keywords

C2C12 myoblast
Ca influx
Muscle differentiation
Myoblast fusion
N-Methyl-D-aspartate receptor

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2004.10.076

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title = "NMDA receptor-mediated calcium influx plays an essential role in myoblast fusion",

abstract = "Ca 2+ influx is known to be prerequisite for myoblast fusion during skeletal muscle differentiation. Here, we show that the N-methyl-D-aspartate (NMDA) receptor is involved in the Ca 2+ influx of C2C12 myoblasts. NMDA receptor (NR) 1 and NR2D were expressed in the myoblasts during muscle differentiation. Using Ca 2+ imaging analysis, Ca 2+ influx through NRs was directly measured at a single-cell level. l-Glutamate increased myoblast fusion as well as intracellular Ca 2+ levels, and both effects were completely blocked by MK801, a selective antagonist of NRs. Furthermore, treatment with the Ca 2+ ionophore A23187 recovered MK801-mediated inhibition of myoblast fusion. These results suggest that the NRs may play an important role in myoblast fusion by mediating Ca 2+ influx.",

keywords = "C2C12 myoblast, Ca influx, Muscle differentiation, Myoblast fusion, N-Methyl-D-aspartate receptor",

author = "Lee, {Kun Ho} and Park, {Jae Yong} and Kyungjin Kim",

note = "Funding Information: This work was supported by grants from National Research Laboratory and Brain Research Center of the 21st Century Frontier Program in Neuroscience from Korea Ministry of Science and Technology to K. Kim. K.H. Lee and J.Y. Park are supported by BK 21 research fellowship from the Ministry of Education and Human Resources Development. The manuscript was edited by Biomedical English Editing Service, Portland OR 97229, USA.",

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doi = "10.1016/j.febslet.2004.10.076",

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AU - Lee, Kun Ho

AU - Park, Jae Yong

AU - Kim, Kyungjin

N1 - Funding Information: This work was supported by grants from National Research Laboratory and Brain Research Center of the 21st Century Frontier Program in Neuroscience from Korea Ministry of Science and Technology to K. Kim. K.H. Lee and J.Y. Park are supported by BK 21 research fellowship from the Ministry of Education and Human Resources Development. The manuscript was edited by Biomedical English Editing Service, Portland OR 97229, USA.

PY - 2004/12/3

Y1 - 2004/12/3

N2 - Ca 2+ influx is known to be prerequisite for myoblast fusion during skeletal muscle differentiation. Here, we show that the N-methyl-D-aspartate (NMDA) receptor is involved in the Ca 2+ influx of C2C12 myoblasts. NMDA receptor (NR) 1 and NR2D were expressed in the myoblasts during muscle differentiation. Using Ca 2+ imaging analysis, Ca 2+ influx through NRs was directly measured at a single-cell level. l-Glutamate increased myoblast fusion as well as intracellular Ca 2+ levels, and both effects were completely blocked by MK801, a selective antagonist of NRs. Furthermore, treatment with the Ca 2+ ionophore A23187 recovered MK801-mediated inhibition of myoblast fusion. These results suggest that the NRs may play an important role in myoblast fusion by mediating Ca 2+ influx.

AB - Ca 2+ influx is known to be prerequisite for myoblast fusion during skeletal muscle differentiation. Here, we show that the N-methyl-D-aspartate (NMDA) receptor is involved in the Ca 2+ influx of C2C12 myoblasts. NMDA receptor (NR) 1 and NR2D were expressed in the myoblasts during muscle differentiation. Using Ca 2+ imaging analysis, Ca 2+ influx through NRs was directly measured at a single-cell level. l-Glutamate increased myoblast fusion as well as intracellular Ca 2+ levels, and both effects were completely blocked by MK801, a selective antagonist of NRs. Furthermore, treatment with the Ca 2+ ionophore A23187 recovered MK801-mediated inhibition of myoblast fusion. These results suggest that the NRs may play an important role in myoblast fusion by mediating Ca 2+ influx.

KW - C2C12 myoblast

KW - Ca influx

KW - Muscle differentiation

KW - Myoblast fusion

KW - N-Methyl-D-aspartate receptor

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ER -

NMDA receptor-mediated calcium influx plays an essential role in myoblast fusion

Abstract

Keywords

ASJC Scopus subject areas

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