Abstract
Ca 2+ influx is known to be prerequisite for myoblast fusion during skeletal muscle differentiation. Here, we show that the N-methyl-D-aspartate (NMDA) receptor is involved in the Ca 2+ influx of C2C12 myoblasts. NMDA receptor (NR) 1 and NR2D were expressed in the myoblasts during muscle differentiation. Using Ca 2+ imaging analysis, Ca 2+ influx through NRs was directly measured at a single-cell level. l-Glutamate increased myoblast fusion as well as intracellular Ca 2+ levels, and both effects were completely blocked by MK801, a selective antagonist of NRs. Furthermore, treatment with the Ca 2+ ionophore A23187 recovered MK801-mediated inhibition of myoblast fusion. These results suggest that the NRs may play an important role in myoblast fusion by mediating Ca 2+ influx.
Original language | English |
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Pages (from-to) | 47-52 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 578 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 2004 Dec 3 |
Externally published | Yes |
Keywords
- C2C12 myoblast
- Ca influx
- Muscle differentiation
- Myoblast fusion
- N-Methyl-D-aspartate receptor
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology