Abstract
Ca 2+ influx is known to be prerequisite for myoblast fusion during skeletal muscle differentiation. Here, we show that the N-methyl-D-aspartate (NMDA) receptor is involved in the Ca 2+ influx of C2C12 myoblasts. NMDA receptor (NR) 1 and NR2D were expressed in the myoblasts during muscle differentiation. Using Ca 2+ imaging analysis, Ca 2+ influx through NRs was directly measured at a single-cell level. l-Glutamate increased myoblast fusion as well as intracellular Ca 2+ levels, and both effects were completely blocked by MK801, a selective antagonist of NRs. Furthermore, treatment with the Ca 2+ ionophore A23187 recovered MK801-mediated inhibition of myoblast fusion. These results suggest that the NRs may play an important role in myoblast fusion by mediating Ca 2+ influx.
| Original language | English |
|---|---|
| Pages (from-to) | 47-52 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 578 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2004 Dec 3 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by grants from National Research Laboratory and Brain Research Center of the 21st Century Frontier Program in Neuroscience from Korea Ministry of Science and Technology to K. Kim. K.H. Lee and J.Y. Park are supported by BK 21 research fellowship from the Ministry of Education and Human Resources Development. The manuscript was edited by Biomedical English Editing Service, Portland OR 97229, USA.
Keywords
- C2C12 myoblast
- Ca influx
- Muscle differentiation
- Myoblast fusion
- N-Methyl-D-aspartate receptor
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology