NO inhibitory phytochemicals as potential anti-inflammatory agents from the twigs of Trigonostemon heterophyllus

Yaru Xi, Lijun An, Xueyuan Yang, Ziteng Song, Jie Zhang, Muhetaer Tuerhong, Da Qing Jin, Yasushi Ohizumi, Dongho Lee, Jing Xu, Yuanqiang Guo

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)

    Abstract

    Studies on the relationship of nitric oxide (NO) and inflammation have revealed that compounds with NO inhibitory effects are potentially useful for inflammation and related inflammatory disorders. A phytochemical investigation to obtain new NO inhibitors resulted in the isolation of two new cleistanthane diterpenoids (1 and 2) and 11 known terpenoids (3–13) from Trigonostemon heterophyllus. The structures of these terpenoids were established by analysis of their NMR, MS, and electronic circular dichroism (ECD) data. Compounds 1 and 2 possess rare 3,4-seco-cleistanthane diterpenoid skeletons. All of the isolates were evaluated biologically for their NO inhibitory effects in lipopolysaccharide (LPS)-induced murine microglial BV-2 cells and compounds 1, 6, and 8–10 showed strong NO inhibitory effects with IC 50 values less than 40 μM. Using Western blotting experiments and molecular docking, the possible mechanism of NO inhibition was investigated.

    Original languageEnglish
    Pages (from-to)417-424
    Number of pages8
    JournalBioorganic Chemistry
    Volume87
    DOIs
    Publication statusPublished - 2019 Jun

    Bibliographical note

    Funding Information:
    This research was supported by the National Key Research and Development Program of China (No. 2018YFA0507204), Hundred Young Academic Leaders Program of Nankai University, the Natural Science Foundation of Tianjin, China (No. 16JCYBJC27700), National Natural Science Foundation of China (Nos. U1703107, 21642016, and 21372125), and State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University, No. CMEMR2018-B02).

    Funding Information:
    This research was supported by the National Key Research and Development Program of China (No. 2018YFA0507204 ), Hundred Young Academic Leaders Program of Nankai University, the Natural Science Foundation of Tianjin, China (No. 16JCYBJC27700 ), National Natural Science Foundation of China (Nos. U1703107 , 21642016 , and 21372125 ), and State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University, No. CMEMR2018-B02 ).

    Publisher Copyright:
    © 2019 Elsevier Inc.

    Keywords

    • Cleistanthane diterpenoids
    • Inflammation
    • Molecular docking
    • NO inhibitors
    • Trigonostemon heterophyllus

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Drug Discovery
    • Organic Chemistry

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