Nocardioides litoris sp. nov., isolated from the taean seashore

A novel Gram-stain-positive, irregularly rod-shaped actinomycete, designated strain 002-2^T, was isolated from sand beach sediment collected from the Taean seashore, Republic of Korea. Cells were aerobic, oxidase-negative and catalase-positive. Colonies of cells were bright yellow, circular, smooth and convex. The diagnostic diamino acid in the cell wall was LLdiaminopimelic acid. The predominant menaquinone was MK-8(H₄). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid and two unknown amino phospholipids. The major fatty acids were iso-C_16:0 and C_17:1ѡ8c. The DNA G+C content was 68.8 mol%. 16S rRNA gene sequence analysis revealed that strain 002-2^T belongs to the family Nocardioidaceae and formed a cluster with Nocardioides rubroscoriae Sco- A25^T (98.1% sequence similarity) and Nocardioides plantarum NCIMB 12834^T (97.6 %). On the basis of the phenotypic and phylogenetic data, strain 002-2^T (=KCTC 39838^T=DSM 103718^T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides litoris sp. nov. is proposed.