Non-Photosynthetic CO2Utilization to Increase Fatty Acid Production in Yarrowia lipolytica

Seung Kyou You; Hyeon Min Park; Myeong Eun Lee; Young Jin Ko; Dong Hyeuk Hwang; Jun Won Oh; Sung Ok Han

doi:10.1021/acs.jafc.1c04308

Non-Photosynthetic CO₂Utilization to Increase Fatty Acid Production in Yarrowia lipolytica

Seung Kyou You, Hyeon Min Park, Myeong Eun Lee, Young Jin Ko, Dong Hyeuk Hwang, Jun Won Oh, Sung Ok Han

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Metabolic engineering of non-photosynthetic microorganisms to increase the utilization of CO2 has been focused on as a green strategy to convert CO2 into valuable products such as fatty acids. In this study, a CO2 utilization pathway involving carbonic anhydrase and biotin carboxylase was formed to recycle CO2 in the oleaginous yeast Yarrowia lipolytica, thereby increasing the production of fatty acids. In the recombinant strain in which the CO2 utilization pathway was introduced, the production of fatty acids was 10.7 g/L, which was 1.5-fold higher than that of the wild-type strain. The resulting strain had a 1.4-fold increase in dry cell mass compared to the wild-type strain. In addition, linoleic acid was 47.7% in the fatty acid composition of the final strain, which was increased by 11.6% compared to the wild-type strain. These results can be applied as an essential technology for developing efficient and eco-friendly processes by directly utilizing CO2.

Original language	English
Pages (from-to)	11912-11918
Number of pages	7
Journal	Journal of agricultural and food chemistry
Volume	69
Issue number	40
DOIs	https://doi.org/10.1021/acs.jafc.1c04308
Publication status	Published - 2021 Oct 13

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (no. 2021R1A2C2006944), and supported by an OJeong Resilience Institute (OJERI) and Korea University grant.

Publisher Copyright:
© 2021 American Chemical Society.

Keywords

COutilization
Yarrowia lipolytica
biotin carboxylase
carbonic anhydrase
non-photosynthetic

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

Access to Document

10.1021/acs.jafc.1c04308

Cite this

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title = "Non-Photosynthetic CO2Utilization to Increase Fatty Acid Production in Yarrowia lipolytica",

abstract = "Metabolic engineering of non-photosynthetic microorganisms to increase the utilization of CO2 has been focused on as a green strategy to convert CO2 into valuable products such as fatty acids. In this study, a CO2 utilization pathway involving carbonic anhydrase and biotin carboxylase was formed to recycle CO2 in the oleaginous yeast Yarrowia lipolytica, thereby increasing the production of fatty acids. In the recombinant strain in which the CO2 utilization pathway was introduced, the production of fatty acids was 10.7 g/L, which was 1.5-fold higher than that of the wild-type strain. The resulting strain had a 1.4-fold increase in dry cell mass compared to the wild-type strain. In addition, linoleic acid was 47.7% in the fatty acid composition of the final strain, which was increased by 11.6% compared to the wild-type strain. These results can be applied as an essential technology for developing efficient and eco-friendly processes by directly utilizing CO2. ",

keywords = "COutilization, Yarrowia lipolytica, biotin carboxylase, carbonic anhydrase, non-photosynthetic",

author = "You, {Seung Kyou} and Park, {Hyeon Min} and Lee, {Myeong Eun} and Ko, {Young Jin} and Hwang, {Dong Hyeuk} and Oh, {Jun Won} and Han, {Sung Ok}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (no. 2021R1A2C2006944), and supported by an OJeong Resilience Institute (OJERI) and Korea University grant. Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",

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AU - You, Seung Kyou

AU - Park, Hyeon Min

AU - Lee, Myeong Eun

AU - Ko, Young Jin

AU - Hwang, Dong Hyeuk

AU - Oh, Jun Won

AU - Han, Sung Ok

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (no. 2021R1A2C2006944), and supported by an OJeong Resilience Institute (OJERI) and Korea University grant. Publisher Copyright: © 2021 American Chemical Society.

PY - 2021/10/13

Y1 - 2021/10/13

N2 - Metabolic engineering of non-photosynthetic microorganisms to increase the utilization of CO2 has been focused on as a green strategy to convert CO2 into valuable products such as fatty acids. In this study, a CO2 utilization pathway involving carbonic anhydrase and biotin carboxylase was formed to recycle CO2 in the oleaginous yeast Yarrowia lipolytica, thereby increasing the production of fatty acids. In the recombinant strain in which the CO2 utilization pathway was introduced, the production of fatty acids was 10.7 g/L, which was 1.5-fold higher than that of the wild-type strain. The resulting strain had a 1.4-fold increase in dry cell mass compared to the wild-type strain. In addition, linoleic acid was 47.7% in the fatty acid composition of the final strain, which was increased by 11.6% compared to the wild-type strain. These results can be applied as an essential technology for developing efficient and eco-friendly processes by directly utilizing CO2.

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