Non-specific protein removal and specific protein capture simultaneously using a hydrodynamic force induced under vortex flow

Hanzhe Liu, Dong June Ahn

Research output: Contribution to journalArticlepeer-review

Abstract

Non-specific binding (NSB) of proteins should be removed to increase the sensitivity of biosensors. In this study, cadmium selenide nanoparticles (CdSe NPs) were rotated in the solution by generating vortex flow, thereby achieved removal of two types of non-specific protein: (i) anti-mouse IgG-FITC (NSB) and (ii) bovine serum albumin (BSA)-FITC. By manipulating rotation rate from 0 to 1000 rpm, photoluminescence (PL) from each protein reduced up to 28% for anti-mouse IgG and 35% for BSA, respectively. In addition, capturing specific proteins from mixture solutions enhanced by 41% upon cyclic-mode vortexing, which is 2.7 times higher than conventional continuous vortexing. These results can open up a new feasibility for increasing sensitivity of biosensors just by utilizing properly the hydrodynamic force. Graphical abstract: [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalMacromolecular Research
Volume31
Issue number2
DOIs
Publication statusPublished - 2023 Feb

Bibliographical note

Funding Information:
This work was supported by National Research Foundation of Korea (NRF-2021R1A2C3009955), and a Korea University Grant.

Publisher Copyright:
© 2023, The Author(s), under exclusive licence to The Polymer Society of Korea.

Keywords

  • CdSe nanoparticles
  • Hydrodynamic force
  • Non-specific protein removal
  • Protein capture

ASJC Scopus subject areas

  • General Chemical Engineering
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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