Nonimmunogenetic Viral Capsid Carrier with Cancer Targeting Activity

Bo Ram Lee, Eunji Jo, Hong Yeol Yoon, Chul Joo Yoon, Hyo Jung Lee, Koo Chul Kwon, Tae Woo Kim, Jeewon Lee

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Although protein nanoparticles (PNPs) (e.g., viral capsids) capable of delivering a broad range of drug agents have shown distinctive advantages over synthetic nanomaterials, PNPs have an intrinsic drawback that hampers their clinical application, that is, potential immunogenicity. Here, a novel method for resolving the immunogenicity problem of PNPs, which is based on the genetic presentation of albumin-binding peptides (ABPs) on the surface of PNP, is reported. ABPs are inserted into the surface of a viral capsid (hepatitis B virus capsid/HBVC) while preserving the native self-assembly function of HBVC. The ABPs effectively gather human serum albumins around HBVC and significantly reduce both inflammatory response and immunoglobulin titer in live mice compared to ABP-free HBVC. Furthermore, ABP-conjugated HBVCs remain within tumors for a longer period than HBVCs conjugated to tumor cell receptor-bindingpeptides, indicating that the ABPs are also capable of enhancing tumor-targeting performance. Although applied to HBVC for proof of concept, this novel approach may provide a general platform for resolving immunogenicity and cancer-targeting problems of PNPs, which enables the development of a variety of PNP-based drug delivery carriers with high safety and efficacy.

Original languageEnglish
Article number1800494
JournalAdvanced Science
Volume5
Issue number8
DOIs
Publication statusPublished - 2018 Aug

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF): the 2015 NLRL (National Leading Research Lab.) Project (Grant No. NRF-2015R1A2A1A05001861) and the Bio & Medical Technology Development Program (Grant No. NRF-2017M3A9F5032628).

Publisher Copyright:
© 2018 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

  • albumin
  • albumin-binding peptides
  • immunogenicity
  • protein nanoparticles
  • tumor targeting
  • viral capsids

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • General Chemical Engineering
  • General Materials Science
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • General Engineering
  • General Physics and Astronomy

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