Noninvasive Transcranial Stimulation of Rat Abducens Nerve by Focused Ultrasound

Hyungmin Kim; Seyed Javid Taghados; Krisztina Fischer; Lee So Maeng; Shinsuk Park; Seung Schik Yoo

doi:10.1016/j.ultrasmedbio.2012.04.023

Noninvasive Transcranial Stimulation of Rat Abducens Nerve by Focused Ultrasound

Hyungmin Kim, Seyed Javid Taghados, Krisztina Fischer, Lee So Maeng, Shinsuk Park, Seung Schik Yoo

Research output: Contribution to journal › Article › peer-review

107 Citations (Scopus)

Abstract

Nonpharmacologic and nonsurgical transcranial modulation of the nerve function may provide new opportunities in evaluation and treatment of cranial nerve diseases. This study investigates the possibility of using low-intensity transcranial focused ultrasound (FUS) to selectively stimulate the rat abducens nerve located above the base of the skull. FUS (frequencies of 350 kHz and 650 kHz) operating in a pulsed mode was applied to the abducens nerve of Sprague-Dawley rats under stereotactic guidance. The abductive eyeball movement ipsilateral to the side of sonication was observed at 350 kHz, using the 0.36-msec tone burst duration (TBD), 1.5-kHz pulse repetition frequency (PRF), and the overall sonication duration of 200 msec. Histologic and behavioral monitoring showed no signs of disruption in the blood brain barrier (BBB), as well as no damage to the nerves and adjacent brain tissue resulting from the sonication. As a novel functional neuro-modulatory modality, the pulsed application of FUS has potential for diagnostic and therapeutic applications in diseases of the peripheral nervous system.

Original language	English
Pages (from-to)	1568-1575
Number of pages	8
Journal	Ultrasound in Medicine and Biology
Volume	38
Issue number	9
DOIs	https://doi.org/10.1016/j.ultrasmedbio.2012.04.023
Publication status	Published - 2012 Sept

Bibliographical note

Funding Information:
This work was supported by grants from the National Institute of Health ( R21 NS074124 to Yoo) and the National Research Foundation of Korea (Korean Ministry of Education, Science and Technology, 2010-0027294 to Park). The authors gratefully acknowledge the assistance of Drs. Yong-Zhi Zhang and Byoung-Kyong Min in data acquisition and animal preparation. The authors also thank Alan Chiu for editorial support.

Keywords

Abducens nerve
Brain
Cranial nerve VI
Focused ultrasound
Neuromodulation
Peripheral nervous system

ASJC Scopus subject areas

Radiological and Ultrasound Technology
Biophysics
Acoustics and Ultrasonics

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10.1016/j.ultrasmedbio.2012.04.023

Cite this

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title = "Noninvasive Transcranial Stimulation of Rat Abducens Nerve by Focused Ultrasound",

abstract = "Nonpharmacologic and nonsurgical transcranial modulation of the nerve function may provide new opportunities in evaluation and treatment of cranial nerve diseases. This study investigates the possibility of using low-intensity transcranial focused ultrasound (FUS) to selectively stimulate the rat abducens nerve located above the base of the skull. FUS (frequencies of 350 kHz and 650 kHz) operating in a pulsed mode was applied to the abducens nerve of Sprague-Dawley rats under stereotactic guidance. The abductive eyeball movement ipsilateral to the side of sonication was observed at 350 kHz, using the 0.36-msec tone burst duration (TBD), 1.5-kHz pulse repetition frequency (PRF), and the overall sonication duration of 200 msec. Histologic and behavioral monitoring showed no signs of disruption in the blood brain barrier (BBB), as well as no damage to the nerves and adjacent brain tissue resulting from the sonication. As a novel functional neuro-modulatory modality, the pulsed application of FUS has potential for diagnostic and therapeutic applications in diseases of the peripheral nervous system.",

keywords = "Abducens nerve, Brain, Cranial nerve VI, Focused ultrasound, Neuromodulation, Peripheral nervous system",

author = "Hyungmin Kim and Taghados, {Seyed Javid} and Krisztina Fischer and Maeng, {Lee So} and Shinsuk Park and Yoo, {Seung Schik}",

note = "Funding Information: This work was supported by grants from the National Institute of Health ( R21 NS074124 to Yoo) and the National Research Foundation of Korea (Korean Ministry of Education, Science and Technology, 2010-0027294 to Park). The authors gratefully acknowledge the assistance of Drs. Yong-Zhi Zhang and Byoung-Kyong Min in data acquisition and animal preparation. The authors also thank Alan Chiu for editorial support. ",

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AU - Park, Shinsuk

AU - Yoo, Seung Schik

N1 - Funding Information: This work was supported by grants from the National Institute of Health ( R21 NS074124 to Yoo) and the National Research Foundation of Korea (Korean Ministry of Education, Science and Technology, 2010-0027294 to Park). The authors gratefully acknowledge the assistance of Drs. Yong-Zhi Zhang and Byoung-Kyong Min in data acquisition and animal preparation. The authors also thank Alan Chiu for editorial support.

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N2 - Nonpharmacologic and nonsurgical transcranial modulation of the nerve function may provide new opportunities in evaluation and treatment of cranial nerve diseases. This study investigates the possibility of using low-intensity transcranial focused ultrasound (FUS) to selectively stimulate the rat abducens nerve located above the base of the skull. FUS (frequencies of 350 kHz and 650 kHz) operating in a pulsed mode was applied to the abducens nerve of Sprague-Dawley rats under stereotactic guidance. The abductive eyeball movement ipsilateral to the side of sonication was observed at 350 kHz, using the 0.36-msec tone burst duration (TBD), 1.5-kHz pulse repetition frequency (PRF), and the overall sonication duration of 200 msec. Histologic and behavioral monitoring showed no signs of disruption in the blood brain barrier (BBB), as well as no damage to the nerves and adjacent brain tissue resulting from the sonication. As a novel functional neuro-modulatory modality, the pulsed application of FUS has potential for diagnostic and therapeutic applications in diseases of the peripheral nervous system.

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Noninvasive Transcranial Stimulation of Rat Abducens Nerve by Focused Ultrasound

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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