Nonspecific adsorption minimized nanomechanical immunoassay for application to assay using the real sample

Kyo Seon Hwang; Sang Myung Lee; Byung Hak Cha; Sang Kyung Kim; Jung Ho Park; Tae Song Kim

Nonspecific adsorption minimized nanomechanical immunoassay for application to assay using the real sample

Kyo Seon Hwang, Sang Myung Lee, Byung Hak Cha, Sang Kyung Kim, Jung Ho Park, Tae Song Kim

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We report an application feasibility of the dynamic microcantilever to detect the specific protein in human serum. The mechanical response (i.e. resonant frequency) change of functionalized dynamic microcantilever generated by biomolecular interactions allows one to detect the biomolecules of small amount without labeling process. We implemented the label-free detection of PSA in human serum like sample (protein mixture sample). The range of detected PSA was 1 and 10 ng/ml in clinical concentration range. It is suggest that the dynamic microcantilever enables one to apply the sensitive label-free detection of disease marker proteins in a real human sample.

Original language	English
Title of host publication	Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Editors	Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
Publisher	Chemical and Biological Microsystems Society
Pages	1267-1269
Number of pages	3
ISBN (Electronic)	9780979806407
Publication status	Published - 2007
Event	11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France Duration: 2007 Oct 7 → 2007 Oct 11

Publication series

Name	Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference	11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Country/Territory	France
City	Paris
Period	07/10/7 → 07/10/11

Bibliographical note

Funding Information:
The authors are grateful for the financial support by Intelligent Microsystem Center sponsored by Korea iM nistry of Science and Technology as a part of the 21 st century’s Frontier &DR Projects (Grant SM -01-133-01) and National Core eR search Center for Nanomedical Technology sponsored by KOSEF (Grant 1R 5-2004-024-00000-0).

Publisher Copyright:
© 2007 CBMS.

Keywords

Clinical range
Microcantilever
PSA
Real sample

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Hwang, K. S., Lee, S. M., Cha, B. H., Kim, S. K., Park, J. H., & Kim, T. S. (2007). Nonspecific adsorption minimized nanomechanical immunoassay for application to assay using the real sample. In J.-L. Viovy, P. Tabeling, S. Descroix, & L. Malaquin (Eds.), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 (pp. 1267-1269). (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007). Chemical and Biological Microsystems Society.

Nonspecific adsorption minimized nanomechanical immunoassay for application to assay using the real sample. / Hwang, Kyo Seon; Lee, Sang Myung; Cha, Byung Hak et al.
Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. ed. / Jean-Louis Viovy; Patrick Tabeling; Stephanie Descroix; Laurent Malaquin. Chemical and Biological Microsystems Society, 2007. p. 1267-1269 (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hwang, KS, Lee, SM, Cha, BH, Kim, SK, Park, JH & Kim, TS 2007, Nonspecific adsorption minimized nanomechanical immunoassay for application to assay using the real sample. in J-L Viovy, P Tabeling, S Descroix & L Malaquin (eds), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007, Chemical and Biological Microsystems Society, pp. 1267-1269, 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007, Paris, France, 07/10/7.

Hwang KS, Lee SM, Cha BH, Kim SK, Park JH, Kim TS. Nonspecific adsorption minimized nanomechanical immunoassay for application to assay using the real sample. In Viovy JL, Tabeling P, Descroix S, Malaquin L, editors, Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. Chemical and Biological Microsystems Society. 2007. p. 1267-1269. (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007).

Hwang, Kyo Seon ; Lee, Sang Myung ; Cha, Byung Hak et al. / Nonspecific adsorption minimized nanomechanical immunoassay for application to assay using the real sample. Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. editor / Jean-Louis Viovy ; Patrick Tabeling ; Stephanie Descroix ; Laurent Malaquin. Chemical and Biological Microsystems Society, 2007. pp. 1267-1269 (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007).

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AB - We report an application feasibility of the dynamic microcantilever to detect the specific protein in human serum. The mechanical response (i.e. resonant frequency) change of functionalized dynamic microcantilever generated by biomolecular interactions allows one to detect the biomolecules of small amount without labeling process. We implemented the label-free detection of PSA in human serum like sample (protein mixture sample). The range of detected PSA was 1 and 10 ng/ml in clinical concentration range. It is suggest that the dynamic microcantilever enables one to apply the sensitive label-free detection of disease marker proteins in a real human sample.

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Nonspecific adsorption minimized nanomechanical immunoassay for application to assay using the real sample

Abstract

Publication series

Conference

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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Cite this