TY - JOUR
T1 - Novel platform for minimizing cell loss on separation process
T2 - Droplet-based magnetically activated cell separator
AU - Kim, Youngho
AU - Hong, Su
AU - Lee, Sang Ho
AU - Lee, Kangsun
AU - Yun, Seok
AU - Kang, Yuri
AU - Paek, Kyeong Kap
AU - Ju, Byeong Kwon
AU - Kim, Byungkyu
PY - 2007
Y1 - 2007
N2 - To reduce the problem of cell loss due to adhesion, one of the basic phenomena in microchannel, we proposed the droplet-based magnetically activated cell separator (DMACS). Based on the platform of the DMACS-which consists of permanent magnets, a coverslip with a circle-shaped boundary, and an injection tube-we could collect magnetically (CD45)-labeled (positive) cells with high purity and minimize cell loss due to adhesion. To compare separation efficiency between the MACS and the DMACS, the total number of cells before and after separation with both the separators was counted by flow cytometry. We could find that the number (324159 940) of cells lost in the DMACS is much less than that (22 36059 940) in the MACS while the efficiency of cell separation in the DMACS (96.07%) is almost the same as that in the MACS (96.72%). Practically, with fluorescent images, it was visually confirmed that the statistical data are reliable. From the viability test by using Hoechst 33 342, it was also demonstrated that there was no cell damage on a gas-liquid interface. Conclusively, DMACS will be a powerful tool to separate rare cells and applicable as a separator, key component of lab-on-a-chip.
AB - To reduce the problem of cell loss due to adhesion, one of the basic phenomena in microchannel, we proposed the droplet-based magnetically activated cell separator (DMACS). Based on the platform of the DMACS-which consists of permanent magnets, a coverslip with a circle-shaped boundary, and an injection tube-we could collect magnetically (CD45)-labeled (positive) cells with high purity and minimize cell loss due to adhesion. To compare separation efficiency between the MACS and the DMACS, the total number of cells before and after separation with both the separators was counted by flow cytometry. We could find that the number (324159 940) of cells lost in the DMACS is much less than that (22 36059 940) in the MACS while the efficiency of cell separation in the DMACS (96.07%) is almost the same as that in the MACS (96.72%). Practically, with fluorescent images, it was visually confirmed that the statistical data are reliable. From the viability test by using Hoechst 33 342, it was also demonstrated that there was no cell damage on a gas-liquid interface. Conclusively, DMACS will be a powerful tool to separate rare cells and applicable as a separator, key component of lab-on-a-chip.
UR - http://www.scopus.com/inward/record.url?scp=34547573326&partnerID=8YFLogxK
U2 - 10.1063/1.2751414
DO - 10.1063/1.2751414
M3 - Article
C2 - 17672779
AN - SCOPUS:34547573326
SN - 0034-6748
VL - 78
JO - Review of Scientific Instruments
JF - Review of Scientific Instruments
IS - 7
M1 - 074301
ER -