Novel self-assembly-induced gelation for nanofibrous collagen/hydroxyapatite composite microspheres

Jae Won Choi; Jong Woo Kim; In Hwan Jo; Young Hag Koh; Hyoun Ee Kim

doi:10.3390/ma10101110

Novel self-assembly-induced gelation for nanofibrous collagen/hydroxyapatite composite microspheres

Jae Won Choi, Jong Woo Kim, In Hwan Jo, Young Hag Koh, Hyoun Ee Kim

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

This study demonstrates the utility of the newly developed self-assembly-induced gelation technique for the synthesis of porous collagen/hydroxyapatite (HA) composite microspheres with a nanofibrous structure. This new approach can produce microspheres of a uniform size using the droplets that form at the nozzle tip before gelation. These microspheres can have a highly nanofibrous structure due to the immersion of the droplets in a coagulation bath (water/acetone), in which the collagen aggregates in the solution can self-assemble into fibrils due to pH-dependent precipitation. Bioactive HA particles were incorporated into the collagen solutions, in order to enhance the bioactivity of the composite microspheres. The composite microspheres exhibited a well-defined spherical morphology and a uniform size for all levels of HA content (0 wt %, 10 wt %, 15 wt %, and 20 wt %). Collagen nanofibers-several tens of nanometers in size-were uniformly present throughout the microspheres and the HA particles were also well dispersed. The in vitro apatite-forming ability, assessed using the simulated body fluid (SBF) solution, increased significantly with the incorporation of HA into the composite microspheres.

Original language	English
Article number	1110
Journal	Materials
Volume	10
Issue number	10
DOIs	https://doi.org/10.3390/ma10101110
Publication status	Published - 2017 Sept 21

Bibliographical note

Funding Information:
4.ConclusionsNanofibrous collagen/HA composite microspheres with different levels of HA content (0 wt %, 10 wtN%a,n1o5fiwbrto%u,sacnodlla2g0ewnt/%H)Awceorme spuocscietessmfuilclryossypnhtehreessizweidthudsiinffge rseenlft-alesvseemls bolfyH-inAdcuocnetdengtel(a0tiwont —%, 1n0ewwlyt d%e,ve1l5opwedt i%n,thainsdstu2d0yw.Atll%th)ewceormepsouscitcee smsfiucrlolyspshyenrtehse hsaizde sdimuislianrg disaemlfe-atesrsse mwbitlhy -ai nndaurrcoewd gsiezleatdioisnt—ribnuetwiolny, draenvgeilnogpebdetiwnetehni s79st8u μdmy. ±A 4ll2tμhme caonmdp 8o0s9i tμemm i±cr3o4spμhme.reIns ahdadditsiiomni, larergdairadmleestseorsf wthiet hHaAn acrornotwensti,z ceodlliasgtreinbu ntiaonno,fribanergsi—ngsebveetwrael etnen7s98ofµ mnan±o4m2eµtemrsa innd s8iz0e9—µwme±re3u4nµifmor.mInlya dcrdeiatitoend, rthegroaurdglhesosuotfththee cHomApcoonsitteenmt, iccorlolasgpehnerneasn. oTfhibee Hrs—A speavretricalletsenwseorfenaalnsoo msuectecersssinfuslilzye i—ncworepreourantiefdor imntloy cthreea cteodmtphorosiuteg hmoiuctrothspehceormesp,o wsihteicmh iscirgonsipfihcearnetsl.yT ehnehHanAcepdarinti cvlietsrowaepraetiatles-ofosrumcciensgs faublillyitiyn.cAornpuomrabteedr ionft aopathtiete ccormysptaolssitveigmoriocruosslyp hperreecsi,piwtahteidch onsi tghnei fsiucarnfatclye oefnthhaen ccoemd pionsvitietr mo iacproastipteh-efroersm aifntegr 3a bdialiytys. Aof nimummbeerrsioofnaipna tainte ScBryFs staollsuvtiiogno.roItuwslaysparlescoi pfiotuatnedd tohnatthheigshuerrfa HceAofcothnetecnotmlepdo stiotehmigihcerorsipnhveirterso abfitoearc3tivdiatyy.s Tohfeimsem fienrdsiinognsi nsuagngSesBtFthsoatlusteilof-na.sIstewmabslya-lisnodfuocuenddgtehlaattihoing choeurlHd Abec uonsetedn ftolre ad vtoarhieigtyh eorf ipnovlyitmroebr isooalcuttiivointys. aTnhde bsee faipnpdliinegds insuag nguesmt bthera tosf edlfif-faesrseenmt bfileyl-dins.d uced gelation could be used for a variety of polymer solutions and be applied in a number of different fields. Acknowledgments: This study was supported by a grant of the Korean Health Technology R&D Project, AMciknnisotrwyl oedf Hgmeaelnthts &: T Whieslsfatured,y Rwepasubsulipc poof rKteodrebay (acognratrnatcotfgtrhaenKt nouremanbeHr:e HalIth13TCe1ch50n1o)l.o gy R&D Project, Ministry of Health & Welfare, Republic of Korea (contract grant number: HI13C1501). Author Contributions: J.W. Choi, Y.H. Koh, and H.E. Kim conceived and designed the experiments; J.W. Choi Author Contributions: J.W. Choi, Y.H. Koh, and H.E. Kim conceived and designed the experiments; J.W. Choi and J.W. Kim synthesized the nanofibrous collagen/HA composite microspheres and evaluated their morphology, morphology, microstructure/nanostructure, crystalline structure, chemical composition, and thermal properties; J.W. Choi and I.H. Jo characterized the apatite-forming ability of the composite microspheres; J.W. Choi and mY.aHn.u Kscorhip wt.rAotlel tthhee amuatnhuorsscrciopnt.t rAibllu ttheed atuotthhoerfsi ncoalnetrdibituintegdo tfot htheemfiannaul secdriiptitn.g of the manuscript.

Publisher Copyright:
© 2017 by the authors.

Keywords

Biomaterials
Collagen
Hydroxyapatite
In vitro bioactivity
Porous scaffolds

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics

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10.3390/ma10101110

Cite this

@article{6a18c0055493431596e21ca9a5b9c87b,

title = "Novel self-assembly-induced gelation for nanofibrous collagen/hydroxyapatite composite microspheres",

abstract = "This study demonstrates the utility of the newly developed self-assembly-induced gelation technique for the synthesis of porous collagen/hydroxyapatite (HA) composite microspheres with a nanofibrous structure. This new approach can produce microspheres of a uniform size using the droplets that form at the nozzle tip before gelation. These microspheres can have a highly nanofibrous structure due to the immersion of the droplets in a coagulation bath (water/acetone), in which the collagen aggregates in the solution can self-assemble into fibrils due to pH-dependent precipitation. Bioactive HA particles were incorporated into the collagen solutions, in order to enhance the bioactivity of the composite microspheres. The composite microspheres exhibited a well-defined spherical morphology and a uniform size for all levels of HA content (0 wt %, 10 wt %, 15 wt %, and 20 wt %). Collagen nanofibers-several tens of nanometers in size-were uniformly present throughout the microspheres and the HA particles were also well dispersed. The in vitro apatite-forming ability, assessed using the simulated body fluid (SBF) solution, increased significantly with the incorporation of HA into the composite microspheres.",

keywords = "Biomaterials, Collagen, Hydroxyapatite, In vitro bioactivity, Porous scaffolds",

author = "Choi, {Jae Won} and Kim, {Jong Woo} and Jo, {In Hwan} and Koh, {Young Hag} and Kim, {Hyoun Ee}",

note = "Funding Information: 4.ConclusionsNanofibrous collagen/HA composite microspheres with different levels of HA content (0 wt %, 10 wtN%a,n1o5fiwbrto%u,sacnodlla2g0ewnt/%H)Awceorme spuocscietessmfuilclryossypnhtehreessizweidthudsiinffge rseenlft-alesvseemls bolfyH-inAdcuocnetdengtel(a0tiwont —%, 1n0ewwlyt d%e,ve1l5opwedt i%n,thainsdstu2d0yw.Atll%th)ewceormepsouscitcee smsfiucrlolyspshyenrtehse hsaizde sdimuislianrg disaemlfe-atesrsse mwbitlhy -ai nndaurrcoewd gsiezleatdioisnt—ribnuetwiolny, draenvgeilnogpebdetiwnetehni s79st8u μdmy. ±A 4ll2tμhme caonmdp 8o0s9i tμemm i±cr3o4spμhme.reIns ahdadditsiiomni, larergdairadmleestseorsf wthiet hHaAn acrornotwensti,z ceodlliasgtreinbu ntiaonno,fribanergsi—ngsebveetwrael etnen7s98ofµ mnan±o4m2eµtemrsa innd s8iz0e9—µwme±re3u4nµifmor.mInlya dcrdeiatitoend, rthegroaurdglhesosuotfththee cHomApcoonsitteenmt, iccorlolasgpehnerneasn. oTfhibee Hrs—A speavretricalletsenwseorfenaalnsoo msuectecersssinfuslilzye i—ncworepreourantiefdor imntloy cthreea cteodmtphorosiuteg hmoiuctrothspehceormesp,o wsihteicmh iscirgonsipfihcearnetsl.yT ehnehHanAcepdarinti cvlietsrowaepraetiatles-ofosrumcciensgs faublillyitiyn.cAornpuomrabteedr ionft aopathtiete ccormysptaolssitveigmoriocruosslyp hperreecsi,piwtahteidch onsi tghnei fsiucarnfatclye oefnthhaen ccoemd pionsvitietr mo iacproastipteh-efroersm aifntegr 3a bdialiytys. Aof nimummbeerrsioofnaipna tainte ScBryFs staollsuvtiiogno.roItuwslaysparlescoi pfiotuatnedd tohnatthheigshuerrfa HceAofcothnetecnotmlepdo stiotehmigihcerorsipnhveirterso abfitoearc3tivdiatyy.s Tohfeimsem fienrdsiinognsi nsuagngSesBtFthsoatlusteilof-na.sIstewmabslya-lisnodfuocuenddgtehlaattihoing choeurlHd Abec uonsetedn ftolre ad vtoarhieigtyh eorf ipnovlyitmroebr isooalcuttiivointys. aTnhde bsee faipnpdliinegds insuag nguesmt bthera tosf edlfif-faesrseenmt bfileyl-dins.d uced gelation could be used for a variety of polymer solutions and be applied in a number of different fields. Acknowledgments: This study was supported by a grant of the Korean Health Technology R&D Project, AMciknnisotrwyl oedf Hgmeaelnthts &: T Whieslsfatured,y Rwepasubsulipc poof rKteodrebay (acognratrnatcotfgtrhaenKt nouremanbeHr:e HalIth13TCe1ch50n1o)l.o gy R&D Project, Ministry of Health & Welfare, Republic of Korea (contract grant number: HI13C1501). Author Contributions: J.W. Choi, Y.H. Koh, and H.E. Kim conceived and designed the experiments; J.W. Choi Author Contributions: J.W. Choi, Y.H. Koh, and H.E. Kim conceived and designed the experiments; J.W. Choi and J.W. Kim synthesized the nanofibrous collagen/HA composite microspheres and evaluated their morphology, morphology, microstructure/nanostructure, crystalline structure, chemical composition, and thermal properties; J.W. Choi and I.H. Jo characterized the apatite-forming ability of the composite microspheres; J.W. Choi and mY.aHn.u Kscorhip wt.rAotlel tthhee amuatnhuorsscrciopnt.t rAibllu ttheed atuotthhoerfsi ncoalnetrdibituintegdo tfot htheemfiannaul secdriiptitn.g of the manuscript. Publisher Copyright: {\textcopyright} 2017 by the authors.",

year = "2017",

month = sep,

day = "21",

doi = "10.3390/ma10101110",

language = "English",

volume = "10",

journal = "Materials",

issn = "1996-1944",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "10",

}

TY - JOUR

T1 - Novel self-assembly-induced gelation for nanofibrous collagen/hydroxyapatite composite microspheres

AU - Choi, Jae Won

AU - Kim, Jong Woo

AU - Jo, In Hwan

AU - Koh, Young Hag

AU - Kim, Hyoun Ee

N1 - Funding Information: 4.ConclusionsNanofibrous collagen/HA composite microspheres with different levels of HA content (0 wt %, 10 wtN%a,n1o5fiwbrto%u,sacnodlla2g0ewnt/%H)Awceorme spuocscietessmfuilclryossypnhtehreessizweidthudsiinffge rseenlft-alesvseemls bolfyH-inAdcuocnetdengtel(a0tiwont —%, 1n0ewwlyt d%e,ve1l5opwedt i%n,thainsdstu2d0yw.Atll%th)ewceormepsouscitcee smsfiucrlolyspshyenrtehse hsaizde sdimuislianrg disaemlfe-atesrsse mwbitlhy -ai nndaurrcoewd gsiezleatdioisnt—ribnuetwiolny, draenvgeilnogpebdetiwnetehni s79st8u μdmy. ±A 4ll2tμhme caonmdp 8o0s9i tμemm i±cr3o4spμhme.reIns ahdadditsiiomni, larergdairadmleestseorsf wthiet hHaAn acrornotwensti,z ceodlliasgtreinbu ntiaonno,fribanergsi—ngsebveetwrael etnen7s98ofµ mnan±o4m2eµtemrsa innd s8iz0e9—µwme±re3u4nµifmor.mInlya dcrdeiatitoend, rthegroaurdglhesosuotfththee cHomApcoonsitteenmt, iccorlolasgpehnerneasn. oTfhibee Hrs—A speavretricalletsenwseorfenaalnsoo msuectecersssinfuslilzye i—ncworepreourantiefdor imntloy cthreea cteodmtphorosiuteg hmoiuctrothspehceormesp,o wsihteicmh iscirgonsipfihcearnetsl.yT ehnehHanAcepdarinti cvlietsrowaepraetiatles-ofosrumcciensgs faublillyitiyn.cAornpuomrabteedr ionft aopathtiete ccormysptaolssitveigmoriocruosslyp hperreecsi,piwtahteidch onsi tghnei fsiucarnfatclye oefnthhaen ccoemd pionsvitietr mo iacproastipteh-efroersm aifntegr 3a bdialiytys. Aof nimummbeerrsioofnaipna tainte ScBryFs staollsuvtiiogno.roItuwslaysparlescoi pfiotuatnedd tohnatthheigshuerrfa HceAofcothnetecnotmlepdo stiotehmigihcerorsipnhveirterso abfitoearc3tivdiatyy.s Tohfeimsem fienrdsiinognsi nsuagngSesBtFthsoatlusteilof-na.sIstewmabslya-lisnodfuocuenddgtehlaattihoing choeurlHd Abec uonsetedn ftolre ad vtoarhieigtyh eorf ipnovlyitmroebr isooalcuttiivointys. aTnhde bsee faipnpdliinegds insuag nguesmt bthera tosf edlfif-faesrseenmt bfileyl-dins.d uced gelation could be used for a variety of polymer solutions and be applied in a number of different fields. Acknowledgments: This study was supported by a grant of the Korean Health Technology R&D Project, AMciknnisotrwyl oedf Hgmeaelnthts &: T Whieslsfatured,y Rwepasubsulipc poof rKteodrebay (acognratrnatcotfgtrhaenKt nouremanbeHr:e HalIth13TCe1ch50n1o)l.o gy R&D Project, Ministry of Health & Welfare, Republic of Korea (contract grant number: HI13C1501). Author Contributions: J.W. Choi, Y.H. Koh, and H.E. Kim conceived and designed the experiments; J.W. Choi Author Contributions: J.W. Choi, Y.H. Koh, and H.E. Kim conceived and designed the experiments; J.W. Choi and J.W. Kim synthesized the nanofibrous collagen/HA composite microspheres and evaluated their morphology, morphology, microstructure/nanostructure, crystalline structure, chemical composition, and thermal properties; J.W. Choi and I.H. Jo characterized the apatite-forming ability of the composite microspheres; J.W. Choi and mY.aHn.u Kscorhip wt.rAotlel tthhee amuatnhuorsscrciopnt.t rAibllu ttheed atuotthhoerfsi ncoalnetrdibituintegdo tfot htheemfiannaul secdriiptitn.g of the manuscript. Publisher Copyright: © 2017 by the authors.

PY - 2017/9/21

Y1 - 2017/9/21

N2 - This study demonstrates the utility of the newly developed self-assembly-induced gelation technique for the synthesis of porous collagen/hydroxyapatite (HA) composite microspheres with a nanofibrous structure. This new approach can produce microspheres of a uniform size using the droplets that form at the nozzle tip before gelation. These microspheres can have a highly nanofibrous structure due to the immersion of the droplets in a coagulation bath (water/acetone), in which the collagen aggregates in the solution can self-assemble into fibrils due to pH-dependent precipitation. Bioactive HA particles were incorporated into the collagen solutions, in order to enhance the bioactivity of the composite microspheres. The composite microspheres exhibited a well-defined spherical morphology and a uniform size for all levels of HA content (0 wt %, 10 wt %, 15 wt %, and 20 wt %). Collagen nanofibers-several tens of nanometers in size-were uniformly present throughout the microspheres and the HA particles were also well dispersed. The in vitro apatite-forming ability, assessed using the simulated body fluid (SBF) solution, increased significantly with the incorporation of HA into the composite microspheres.

AB - This study demonstrates the utility of the newly developed self-assembly-induced gelation technique for the synthesis of porous collagen/hydroxyapatite (HA) composite microspheres with a nanofibrous structure. This new approach can produce microspheres of a uniform size using the droplets that form at the nozzle tip before gelation. These microspheres can have a highly nanofibrous structure due to the immersion of the droplets in a coagulation bath (water/acetone), in which the collagen aggregates in the solution can self-assemble into fibrils due to pH-dependent precipitation. Bioactive HA particles were incorporated into the collagen solutions, in order to enhance the bioactivity of the composite microspheres. The composite microspheres exhibited a well-defined spherical morphology and a uniform size for all levels of HA content (0 wt %, 10 wt %, 15 wt %, and 20 wt %). Collagen nanofibers-several tens of nanometers in size-were uniformly present throughout the microspheres and the HA particles were also well dispersed. The in vitro apatite-forming ability, assessed using the simulated body fluid (SBF) solution, increased significantly with the incorporation of HA into the composite microspheres.

KW - Biomaterials

KW - Collagen

KW - Hydroxyapatite

KW - In vitro bioactivity

KW - Porous scaffolds

UR - http://www.scopus.com/inward/record.url?scp=85029784979&partnerID=8YFLogxK

U2 - 10.3390/ma10101110

DO - 10.3390/ma10101110

M3 - Article

AN - SCOPUS:85029784979

SN - 1996-1944

VL - 10

JO - Materials

JF - Materials

IS - 10

M1 - 1110

ER -

Novel self-assembly-induced gelation for nanofibrous collagen/hydroxyapatite composite microspheres

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ASJC Scopus subject areas

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