Novel self-assembly-induced gelation for nanofibrous collagen/hydroxyapatite composite microspheres

Jae Won Choi, Jong Woo Kim, In Hwan Jo, Young Hag Koh, Hyoun Ee Kim

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    This study demonstrates the utility of the newly developed self-assembly-induced gelation technique for the synthesis of porous collagen/hydroxyapatite (HA) composite microspheres with a nanofibrous structure. This new approach can produce microspheres of a uniform size using the droplets that form at the nozzle tip before gelation. These microspheres can have a highly nanofibrous structure due to the immersion of the droplets in a coagulation bath (water/acetone), in which the collagen aggregates in the solution can self-assemble into fibrils due to pH-dependent precipitation. Bioactive HA particles were incorporated into the collagen solutions, in order to enhance the bioactivity of the composite microspheres. The composite microspheres exhibited a well-defined spherical morphology and a uniform size for all levels of HA content (0 wt %, 10 wt %, 15 wt %, and 20 wt %). Collagen nanofibers-several tens of nanometers in size-were uniformly present throughout the microspheres and the HA particles were also well dispersed. The in vitro apatite-forming ability, assessed using the simulated body fluid (SBF) solution, increased significantly with the incorporation of HA into the composite microspheres.

    Original languageEnglish
    Article number1110
    JournalMaterials
    Volume10
    Issue number10
    DOIs
    Publication statusPublished - 2017 Sept 21

    Bibliographical note

    Funding Information:
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    Publisher Copyright:
    © 2017 by the authors.

    Keywords

    • Biomaterials
    • Collagen
    • Hydroxyapatite
    • In vitro bioactivity
    • Porous scaffolds

    ASJC Scopus subject areas

    • General Materials Science
    • Condensed Matter Physics

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