Novel self-assembly-induced gelation for nanofibrous collagen/hydroxyapatite composite microspheres

Jae Won Choi, Jong Woo Kim, In Hwan Jo, Young Hag Koh, Hyoun Ee Kim

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


This study demonstrates the utility of the newly developed self-assembly-induced gelation technique for the synthesis of porous collagen/hydroxyapatite (HA) composite microspheres with a nanofibrous structure. This new approach can produce microspheres of a uniform size using the droplets that form at the nozzle tip before gelation. These microspheres can have a highly nanofibrous structure due to the immersion of the droplets in a coagulation bath (water/acetone), in which the collagen aggregates in the solution can self-assemble into fibrils due to pH-dependent precipitation. Bioactive HA particles were incorporated into the collagen solutions, in order to enhance the bioactivity of the composite microspheres. The composite microspheres exhibited a well-defined spherical morphology and a uniform size for all levels of HA content (0 wt %, 10 wt %, 15 wt %, and 20 wt %). Collagen nanofibers-several tens of nanometers in size-were uniformly present throughout the microspheres and the HA particles were also well dispersed. The in vitro apatite-forming ability, assessed using the simulated body fluid (SBF) solution, increased significantly with the incorporation of HA into the composite microspheres.

Original languageEnglish
Article number1110
Issue number10
Publication statusPublished - 2017 Sept 21


  • Biomaterials
  • Collagen
  • Hydroxyapatite
  • In vitro bioactivity
  • Porous scaffolds

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics


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