Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate

Hyun Jung Lee; Hyun Sun Lee; Jang Won Choi; Kyung Soo Ra; Jin Man Kim; Hyung Joo Suh

doi:10.1021/jf202686m

Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate

Hyun Jung Lee, Hyun Sun Lee, Jang Won Choi, Kyung Soo Ra, Jin Man Kim, Hyung Joo Suh

School of Biosystem and Biomedical Science

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC ₅₀ = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC ₅₀) were 2.7 and 1.5 mg/mL, respectively.

Original language	English
Pages (from-to)	11522-11525
Number of pages	4
Journal	Journal of agricultural and food chemistry
Volume	59
Issue number	21
DOIs	https://doi.org/10.1021/jf202686m
Publication status	Published - 2011 Nov 9

Keywords

cocoon
diabetes
silk peptide E5K6
α-glucosidase

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/jf202686m

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title = "Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate",

abstract = "Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC 50 = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC 50) were 2.7 and 1.5 mg/mL, respectively.",

keywords = "cocoon, diabetes, silk peptide E5K6, α-glucosidase",

author = "Lee, {Hyun Jung} and Lee, {Hyun Sun} and Choi, {Jang Won} and Ra, {Kyung Soo} and Kim, {Jin Man} and Suh, {Hyung Joo}",

year = "2011",

month = nov,

day = "9",

doi = "10.1021/jf202686m",

language = "English",

volume = "59",

pages = "11522--11525",

journal = "Journal of agricultural and food chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate

AU - Lee, Hyun Jung

AU - Lee, Hyun Sun

AU - Choi, Jang Won

AU - Ra, Kyung Soo

AU - Kim, Jin Man

AU - Suh, Hyung Joo

PY - 2011/11/9

Y1 - 2011/11/9

N2 - Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC 50 = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC 50) were 2.7 and 1.5 mg/mL, respectively.

AB - Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC 50 = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC 50) were 2.7 and 1.5 mg/mL, respectively.

KW - cocoon

KW - diabetes

KW - silk peptide E5K6

KW - α-glucosidase

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U2 - 10.1021/jf202686m

DO - 10.1021/jf202686m

M3 - Article

C2 - 21895007

AN - SCOPUS:80455140651

SN - 0021-8561

VL - 59

SP - 11522

EP - 11525

JO - Journal of agricultural and food chemistry

JF - Journal of agricultural and food chemistry

IS - 21

ER -

Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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