Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate

Hyun Jung Lee; Hyun Sun Lee; Jang Won Choi; Kyung Soo Ra; Jin Man Kim; Hyung Joo Suh

doi:10.1021/jf202686m

Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate

Hyun Jung Lee
, Hyun Sun Lee
, Jang Won Choi
, Kyung Soo Ra
, Jin Man Kim
, Hyung Joo Suh^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC ₅₀ = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC ₅₀) were 2.7 and 1.5 mg/mL, respectively.

Original language	English
Pages (from-to)	11522-11525
Number of pages	4
Journal	Journal of agricultural and food chemistry
Volume	59
Issue number	21
DOIs	https://doi.org/10.1021/jf202686m
Publication status	Published - 2011 Nov 9

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

cocoon
diabetes
silk peptide E5K6
α-glucosidase

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

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10.1021/jf202686m

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@article{07fc83e12fa849f3a1a0f3a7a419642a,

title = "Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate",

abstract = "Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC 50 = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC 50) were 2.7 and 1.5 mg/mL, respectively.",

keywords = "cocoon, diabetes, silk peptide E5K6, α-glucosidase",

author = "Lee, \{Hyun Jung\} and Lee, \{Hyun Sun\} and Choi, \{Jang Won\} and Ra, \{Kyung Soo\} and Kim, \{Jin Man\} and Suh, \{Hyung Joo\}",

year = "2011",

month = nov,

day = "9",

doi = "10.1021/jf202686m",

language = "English",

volume = "59",

pages = "11522--11525",

journal = "Journal of agricultural and food chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "21",

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TY - JOUR

T1 - Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate

AU - Lee, Hyun Jung

AU - Lee, Hyun Sun

AU - Choi, Jang Won

AU - Ra, Kyung Soo

AU - Kim, Jin Man

AU - Suh, Hyung Joo

PY - 2011/11/9

Y1 - 2011/11/9

N2 - Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC 50 = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC 50) were 2.7 and 1.5 mg/mL, respectively.

AB - Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC 50 = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC 50) were 2.7 and 1.5 mg/mL, respectively.

KW - cocoon

KW - diabetes

KW - silk peptide E5K6

KW - α-glucosidase

UR - https://www.scopus.com/pages/publications/80455140651

U2 - 10.1021/jf202686m

DO - 10.1021/jf202686m

M3 - Article

C2 - 21895007

AN - SCOPUS:80455140651

SN - 0021-8561

VL - 59

SP - 11522

EP - 11525

JO - Journal of agricultural and food chemistry

JF - Journal of agricultural and food chemistry

IS - 21

ER -

Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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