Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate

Hyun Jung Lee, Hyun Sun Lee, Jang Won Choi, Kyung Soo Ra, Jin Man Kim, Hyung Joo Suh

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37 Citations (Scopus)


Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC 50 = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC 50) were 2.7 and 1.5 mg/mL, respectively.

Original languageEnglish
Pages (from-to)11522-11525
Number of pages4
JournalJournal of agricultural and food chemistry
Issue number21
Publication statusPublished - 2011 Nov 9


  • cocoon
  • diabetes
  • silk peptide E5K6
  • α-glucosidase

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)


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