Nutrigenomic analysis of Hypolipidemic effects of Agastache rugosa essential oils in HepG2 cells and C57BL/6 mice

Hee Jin Jun; Mi Ja Chung; Kevin Dawson; Raymond L. Rodriguez; Soung Jin Houng; Sung Yun Cho; Jungae Jeun; Jin Young Kim; Kyung Heon Kim; Kuen Woo Park; Cheong Tae Kim; Sung Joon Lee

doi:10.1007/s10068-010-0030-1

Nutrigenomic analysis of Hypolipidemic effects of Agastache rugosa essential oils in HepG2 cells and C57BL/6 mice

Hee Jin Jun, Mi Ja Chung, Kevin Dawson, Raymond L. Rodriguez, Soung Jin Houng, Sung Yun Cho, Jungae Jeun, Jin Young Kim, Kyung Heon Kim, Kuen Woo Park, Cheong Tae Kim, Sung Joon Lee

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Hypolipidemic effects of Agastache rugosa essential oil (AREO) were investigated. Gas chromatographymass spectrometry (GC-MS) analysis showed that the major compound in AREO is limonene (47%, w/w). AREO (1 mg/mL) markedly reduced low density lipoprotein (LDL) oxidation (-93%). After 3-week feeding of AREO, plasma cholesterol (-28%) and triglyceride levels (-26%) were significantly decreased in C57BL/6J mice. Mouse hepatic transcriptome profiling with oligonucleotide microarray revealed that AREO altered the expression of 2,524 genes. Notably, significant reductions in sterol regulatory element binding factor (SREBF)-1 and SREBF- 2 mRNA levels were detected. Protein expression of HMG-CoA reductase, a major target for SREBP-2, was reduced in HepG2 cells (-36%) and in mouse liver (-35 %). AREO also significantly increased mRNA (+40%) and protein (+83%) expression of the LDL receptor in HepG2 cells and mouse liver, respectively. Our findings suggest that AREO may prevent atherosclerosis via the inhibition of LDL oxidation, down-regulation of SREBF-2 and HMG-CoA reductase expression, and up-regulation of LDL receptor expression.

Original language	English
Pages (from-to)	219-227
Number of pages	9
Journal	Food Science and Biotechnology
Volume	19
Issue number	1
DOIs	https://doi.org/10.1007/s10068-010-0030-1
Publication status	Published - 2010 Feb

Bibliographical note

Funding Information:
Acknowledgments This work was supported by a National Research Foundation of Korea Grant funded by the Korean Government (KRF-2006-311-F00131) and by a grant (Code #20080401034049-0090100) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

Keywords

Agastache rugosa (Korean mint)
Cholesterol
Herbal essential oil
Hypocholesterolemic effect

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology

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10.1007/s10068-010-0030-1

Cite this

@article{8c1134ac9d74478eb9664ab049eb4fe7,

title = "Nutrigenomic analysis of Hypolipidemic effects of Agastache rugosa essential oils in HepG2 cells and C57BL/6 mice",

abstract = "Hypolipidemic effects of Agastache rugosa essential oil (AREO) were investigated. Gas chromatographymass spectrometry (GC-MS) analysis showed that the major compound in AREO is limonene (47%, w/w). AREO (1 mg/mL) markedly reduced low density lipoprotein (LDL) oxidation (-93%). After 3-week feeding of AREO, plasma cholesterol (-28%) and triglyceride levels (-26%) were significantly decreased in C57BL/6J mice. Mouse hepatic transcriptome profiling with oligonucleotide microarray revealed that AREO altered the expression of 2,524 genes. Notably, significant reductions in sterol regulatory element binding factor (SREBF)-1 and SREBF- 2 mRNA levels were detected. Protein expression of HMG-CoA reductase, a major target for SREBP-2, was reduced in HepG2 cells (-36%) and in mouse liver (-35 %). AREO also significantly increased mRNA (+40%) and protein (+83%) expression of the LDL receptor in HepG2 cells and mouse liver, respectively. Our findings suggest that AREO may prevent atherosclerosis via the inhibition of LDL oxidation, down-regulation of SREBF-2 and HMG-CoA reductase expression, and up-regulation of LDL receptor expression.",

keywords = "Agastache rugosa (Korean mint), Cholesterol, Herbal essential oil, Hypocholesterolemic effect",

author = "Jun, {Hee Jin} and Chung, {Mi Ja} and Kevin Dawson and Rodriguez, {Raymond L.} and Houng, {Soung Jin} and Cho, {Sung Yun} and Jungae Jeun and Kim, {Jin Young} and Kim, {Kyung Heon} and Park, {Kuen Woo} and Kim, {Cheong Tae} and Lee, {Sung Joon}",

note = "Funding Information: Acknowledgments This work was supported by a National Research Foundation of Korea Grant funded by the Korean Government (KRF-2006-311-F00131) and by a grant (Code #20080401034049-0090100) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.",

year = "2010",

month = feb,

doi = "10.1007/s10068-010-0030-1",

language = "English",

volume = "19",

pages = "219--227",

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T1 - Nutrigenomic analysis of Hypolipidemic effects of Agastache rugosa essential oils in HepG2 cells and C57BL/6 mice

AU - Jun, Hee Jin

AU - Chung, Mi Ja

AU - Dawson, Kevin

AU - Rodriguez, Raymond L.

AU - Houng, Soung Jin

AU - Cho, Sung Yun

AU - Jeun, Jungae

AU - Kim, Jin Young

AU - Kim, Kyung Heon

AU - Park, Kuen Woo

AU - Kim, Cheong Tae

AU - Lee, Sung Joon

N1 - Funding Information: Acknowledgments This work was supported by a National Research Foundation of Korea Grant funded by the Korean Government (KRF-2006-311-F00131) and by a grant (Code #20080401034049-0090100) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

PY - 2010/2

Y1 - 2010/2

N2 - Hypolipidemic effects of Agastache rugosa essential oil (AREO) were investigated. Gas chromatographymass spectrometry (GC-MS) analysis showed that the major compound in AREO is limonene (47%, w/w). AREO (1 mg/mL) markedly reduced low density lipoprotein (LDL) oxidation (-93%). After 3-week feeding of AREO, plasma cholesterol (-28%) and triglyceride levels (-26%) were significantly decreased in C57BL/6J mice. Mouse hepatic transcriptome profiling with oligonucleotide microarray revealed that AREO altered the expression of 2,524 genes. Notably, significant reductions in sterol regulatory element binding factor (SREBF)-1 and SREBF- 2 mRNA levels were detected. Protein expression of HMG-CoA reductase, a major target for SREBP-2, was reduced in HepG2 cells (-36%) and in mouse liver (-35 %). AREO also significantly increased mRNA (+40%) and protein (+83%) expression of the LDL receptor in HepG2 cells and mouse liver, respectively. Our findings suggest that AREO may prevent atherosclerosis via the inhibition of LDL oxidation, down-regulation of SREBF-2 and HMG-CoA reductase expression, and up-regulation of LDL receptor expression.

AB - Hypolipidemic effects of Agastache rugosa essential oil (AREO) were investigated. Gas chromatographymass spectrometry (GC-MS) analysis showed that the major compound in AREO is limonene (47%, w/w). AREO (1 mg/mL) markedly reduced low density lipoprotein (LDL) oxidation (-93%). After 3-week feeding of AREO, plasma cholesterol (-28%) and triglyceride levels (-26%) were significantly decreased in C57BL/6J mice. Mouse hepatic transcriptome profiling with oligonucleotide microarray revealed that AREO altered the expression of 2,524 genes. Notably, significant reductions in sterol regulatory element binding factor (SREBF)-1 and SREBF- 2 mRNA levels were detected. Protein expression of HMG-CoA reductase, a major target for SREBP-2, was reduced in HepG2 cells (-36%) and in mouse liver (-35 %). AREO also significantly increased mRNA (+40%) and protein (+83%) expression of the LDL receptor in HepG2 cells and mouse liver, respectively. Our findings suggest that AREO may prevent atherosclerosis via the inhibition of LDL oxidation, down-regulation of SREBF-2 and HMG-CoA reductase expression, and up-regulation of LDL receptor expression.

KW - Agastache rugosa (Korean mint)

KW - Cholesterol

KW - Herbal essential oil

KW - Hypocholesterolemic effect

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DO - 10.1007/s10068-010-0030-1

M3 - Article

AN - SCOPUS:79957649461

SN - 1226-7708

VL - 19

SP - 219

EP - 227

JO - Food Science and Biotechnology

JF - Food Science and Biotechnology

IS - 1

ER -

Nutrigenomic analysis of Hypolipidemic effects of Agastache rugosa essential oils in HepG2 cells and C57BL/6 mice

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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