O-GlcNAcylation of Sp1 interrupts Sp1 interaction with NF-Y

Kihong Lim; Hyo Ihl Chang

doi:10.1016/j.bbrc.2009.03.075

O-GlcNAcylation of Sp1 interrupts Sp1 interaction with NF-Y

Kihong Lim, Hyo Ihl Chang

* Honorary professors

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

O-linked N-acetylglucosamine (O-GlcNAc), a monosaccharide N-acetylglucosamine addition on nucleocytoplasmic proteins, is abundant in transcription regulators and has been implicated in gene regulation. Sp1 transcription factor is multiply modified by O-GlcNAc within its serine/threonine-rich region and glutamine-rich transactivation domain. In the present study, we show that O-GlcNAc of Sp1 serine/threonine-rich region interrupts a physical interaction between Sp1 and NF-YA, thus inhibiting Sp1-NF-Y cooperative activation of gene transcription. Our results strengthen the notion that O-GlcNAc regulates gene transcription by modulating the protein-protein interaction network among transcription regulatory proteins.

Original language	English
Pages (from-to)	593-597
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	382
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2009.03.075
Publication status	Published - 2009 May 8

Bibliographical note

Funding Information:
We are grateful to Dr. Roberto Mantovani for providing plasmids encoding NF-Y subunits. This work was supported by a Korea University grant.

Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.

Keywords

NF-Y
O-GlcNAc
Sp1
Transcription with NF-Y

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2009.03.075

Cite this

@article{c58be7c59f654735b521db51f33673c7,

title = "O-GlcNAcylation of Sp1 interrupts Sp1 interaction with NF-Y",

abstract = "O-linked N-acetylglucosamine (O-GlcNAc), a monosaccharide N-acetylglucosamine addition on nucleocytoplasmic proteins, is abundant in transcription regulators and has been implicated in gene regulation. Sp1 transcription factor is multiply modified by O-GlcNAc within its serine/threonine-rich region and glutamine-rich transactivation domain. In the present study, we show that O-GlcNAc of Sp1 serine/threonine-rich region interrupts a physical interaction between Sp1 and NF-YA, thus inhibiting Sp1-NF-Y cooperative activation of gene transcription. Our results strengthen the notion that O-GlcNAc regulates gene transcription by modulating the protein-protein interaction network among transcription regulatory proteins.",

keywords = "NF-Y, O-GlcNAc, Sp1, Transcription with NF-Y",

author = "Kihong Lim and Chang, {Hyo Ihl}",

note = "Funding Information: We are grateful to Dr. Roberto Mantovani for providing plasmids encoding NF-Y subunits. This work was supported by a Korea University grant. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2009",

month = may,

day = "8",

doi = "10.1016/j.bbrc.2009.03.075",

language = "English",

volume = "382",

pages = "593--597",

journal = "Biochemical and biophysical research communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - O-GlcNAcylation of Sp1 interrupts Sp1 interaction with NF-Y

AU - Lim, Kihong

AU - Chang, Hyo Ihl

N1 - Funding Information: We are grateful to Dr. Roberto Mantovani for providing plasmids encoding NF-Y subunits. This work was supported by a Korea University grant. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2009/5/8

Y1 - 2009/5/8

N2 - O-linked N-acetylglucosamine (O-GlcNAc), a monosaccharide N-acetylglucosamine addition on nucleocytoplasmic proteins, is abundant in transcription regulators and has been implicated in gene regulation. Sp1 transcription factor is multiply modified by O-GlcNAc within its serine/threonine-rich region and glutamine-rich transactivation domain. In the present study, we show that O-GlcNAc of Sp1 serine/threonine-rich region interrupts a physical interaction between Sp1 and NF-YA, thus inhibiting Sp1-NF-Y cooperative activation of gene transcription. Our results strengthen the notion that O-GlcNAc regulates gene transcription by modulating the protein-protein interaction network among transcription regulatory proteins.

AB - O-linked N-acetylglucosamine (O-GlcNAc), a monosaccharide N-acetylglucosamine addition on nucleocytoplasmic proteins, is abundant in transcription regulators and has been implicated in gene regulation. Sp1 transcription factor is multiply modified by O-GlcNAc within its serine/threonine-rich region and glutamine-rich transactivation domain. In the present study, we show that O-GlcNAc of Sp1 serine/threonine-rich region interrupts a physical interaction between Sp1 and NF-YA, thus inhibiting Sp1-NF-Y cooperative activation of gene transcription. Our results strengthen the notion that O-GlcNAc regulates gene transcription by modulating the protein-protein interaction network among transcription regulatory proteins.

KW - NF-Y

KW - O-GlcNAc

KW - Sp1

KW - Transcription with NF-Y

UR - http://www.scopus.com/inward/record.url?scp=64049092248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64049092248&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.03.075

DO - 10.1016/j.bbrc.2009.03.075

M3 - Article

C2 - 19302979

AN - SCOPUS:64049092248

SN - 0006-291X

VL - 382

SP - 593

EP - 597

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

IS - 3

ER -

O-GlcNAcylation of Sp1 interrupts Sp1 interaction with NF-Y

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this