Oat AsDA1-2D enhances heat stress tolerance and negatively regulates seed-storage globulin

Joo Sun Lee, Chan Seop Ko, Yong Weon Seo

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The importance of oats has increased because of their high nutritional value and health benefits in the human diet. High-temperature stress during the reproductive growth period has a detrimental effect on grain morphology by changing the structure and concentration of several seed-storage proteins. DA1, a conserved ubiquitin-proteasome pathway component, plays an important role in regulating grain size by controlling cell proliferation in maternal integuments during the grain-filling stage. However, there have been no reports or studies on oat DA1 genes. In this study, we identified three DA1-like genes (AsDA1-2D, AsDA1-5A, and AsDA1-1D) using genome-wide analysis. Among these, AsDA1-2D was found to be responsible for high-temperature stress tolerance via a yeast thermotolerance assay. The physical interaction of AsDA1-2D with oat-storage-globulin (AsGL-4D) and a protease inhibitor (AsPI-4D) was observed using yeast two-hybrid screening. A subcellular localization assay revealed that AsDA1-2D and its interacting proteins are localized in the cytosol and plasma membrane. An in vitro pull-down assay showed that AsDA1-2D forms a complex with both AsPI-4D and AsGL-4D. An in vitro cell-free degradation assay showed that AsGL-4D was degraded by AsDA1-2D under high-temperature conditions and that AsPI-4D inhibited the function of AsDA1-2D. These results suggest that AsDA1-2D acts as a cysteine protease and negatively regulates oat-grain-storage-globulin under heat stress.

Original languageEnglish
Article number153981
JournalJournal of Plant Physiology
Volume284
DOIs
Publication statusPublished - 2023 May

Bibliographical note

Funding Information:
This research was funded by the Cooperative Research Program for Agriculture Science & Technology Development ( PJ015705 ), funded by the Rural Development Administration, Republic of Korea . This research was supported by a grant from Korea University, Republic of Korea .

Publisher Copyright:
© 2023

Keywords

  • DA1
  • Globulin
  • Grain-filling period
  • High-temperature stress
  • Oat
  • Protease inhibitor

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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