Occurrence of aflatoxin M1 in Korean dairy products determined by ELISA and HPLC

E. K. Kim; D. H. Shon; D. Ryu; J. W. Park; H. J. Hwang; Y. B. Kim

doi:10.1080/026520300283595

Occurrence of aflatoxin M₁ in Korean dairy products determined by ELISA and HPLC

E. K. Kim
, D. H. Shon
, D. Ryu
, J. W. Park
, H. J. Hwang
, Y. B. Kim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

150 Citations (Scopus)

Abstract

The occurrence of aflatoxin M₁ (AFM₁)in pasteurized milk and dairy products was investigated by using direct competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). The recoveries of AFM₁ from the samples spiked at levels between 5 and 500 pg/ml were 88.0–106.5% for pasteurized milk and 84.0–94.0% for yoghurt by ELISA. By HPLC, the recoveries were 103–120% for pasteurized milk and 87.0–93.0% for yoghurt. The limits of detection were found to be 2 pg/ml by ELISA and 10 pg/ml by HPLC. Among a total of 180 samples collected in Seoul, Korea, the incidence of AFM₁ in pasteurized milk, infant formula, powdered milk and yoghurt was 76, 85, 75, and 83%, respectively, with a mean concentration of 18, 46, 200, and 29 pg/g, respectively, when determined by ELISA. These results obtained by ELISA were closely related to those by HPLC for AFM₁ (r² = 0.9783).

Original language	English
Pages (from-to)	59-64
Number of pages	6
Journal	Food Additives and Contaminants
Volume	17
Issue number	1
DOIs	https://doi.org/10.1080/026520300283595
Publication status	Published - 2000 Jan

Keywords

Aflatoxin
Afm1
Assay
Chromatography
Elisa
Enzyme-linked
High
Hplc
Immunosorbent
Liquid
M1
Performance

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Food Science
General Chemistry
Health, Toxicology and Mutagenesis
Toxicology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1080/026520300283595

Cite this

@article{2f646e6f674a45e599a3c35c08eda368,

title = "Occurrence of aflatoxin M1 in Korean dairy products determined by ELISA and HPLC",

abstract = "The occurrence of aflatoxin M1 (AFM1)in pasteurized milk and dairy products was investigated by using direct competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). The recoveries of AFM1 from the samples spiked at levels between 5 and 500 pg/ml were 88.0–106.5\% for pasteurized milk and 84.0–94.0\% for yoghurt by ELISA. By HPLC, the recoveries were 103–120\% for pasteurized milk and 87.0–93.0\% for yoghurt. The limits of detection were found to be 2 pg/ml by ELISA and 10 pg/ml by HPLC. Among a total of 180 samples collected in Seoul, Korea, the incidence of AFM1 in pasteurized milk, infant formula, powdered milk and yoghurt was 76, 85, 75, and 83\%, respectively, with a mean concentration of 18, 46, 200, and 29 pg/g, respectively, when determined by ELISA. These results obtained by ELISA were closely related to those by HPLC for AFM1 (r2 = 0.9783).",

keywords = "Aflatoxin, Afm1, Assay, Chromatography, Elisa, Enzyme-linked, High, Hplc, Immunosorbent, Liquid, M1, Performance",

author = "Kim, \{E. K.\} and Shon, \{D. H.\} and D. Ryu and Park, \{J. W.\} and Hwang, \{H. J.\} and Kim, \{Y. B.\}",

year = "2000",

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doi = "10.1080/026520300283595",

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T1 - Occurrence of aflatoxin M1 in Korean dairy products determined by ELISA and HPLC

AU - Kim, E. K.

AU - Shon, D. H.

AU - Ryu, D.

AU - Park, J. W.

AU - Hwang, H. J.

AU - Kim, Y. B.

PY - 2000/1

Y1 - 2000/1

N2 - The occurrence of aflatoxin M1 (AFM1)in pasteurized milk and dairy products was investigated by using direct competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). The recoveries of AFM1 from the samples spiked at levels between 5 and 500 pg/ml were 88.0–106.5% for pasteurized milk and 84.0–94.0% for yoghurt by ELISA. By HPLC, the recoveries were 103–120% for pasteurized milk and 87.0–93.0% for yoghurt. The limits of detection were found to be 2 pg/ml by ELISA and 10 pg/ml by HPLC. Among a total of 180 samples collected in Seoul, Korea, the incidence of AFM1 in pasteurized milk, infant formula, powdered milk and yoghurt was 76, 85, 75, and 83%, respectively, with a mean concentration of 18, 46, 200, and 29 pg/g, respectively, when determined by ELISA. These results obtained by ELISA were closely related to those by HPLC for AFM1 (r2 = 0.9783).

AB - The occurrence of aflatoxin M1 (AFM1)in pasteurized milk and dairy products was investigated by using direct competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). The recoveries of AFM1 from the samples spiked at levels between 5 and 500 pg/ml were 88.0–106.5% for pasteurized milk and 84.0–94.0% for yoghurt by ELISA. By HPLC, the recoveries were 103–120% for pasteurized milk and 87.0–93.0% for yoghurt. The limits of detection were found to be 2 pg/ml by ELISA and 10 pg/ml by HPLC. Among a total of 180 samples collected in Seoul, Korea, the incidence of AFM1 in pasteurized milk, infant formula, powdered milk and yoghurt was 76, 85, 75, and 83%, respectively, with a mean concentration of 18, 46, 200, and 29 pg/g, respectively, when determined by ELISA. These results obtained by ELISA were closely related to those by HPLC for AFM1 (r2 = 0.9783).

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KW - Chromatography

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KW - High

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KW - Immunosorbent

KW - Liquid

KW - M1

KW - Performance

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