TY - JOUR
T1 - Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria
AU - Park, Yeon Joon
AU - Park, Sun Young
AU - Oh, Eun Jee
AU - Park, Jung Jun
AU - Lee, Kyo Young
AU - Woo, Gun Jo
AU - Lee, Kyungwon
N1 - Funding Information:
The authors thank all the contributing laboratories that provided isolates for this study. This work was supported by grant from Korea Food and Drug Administration in 2003.
PY - 2005/4
Y1 - 2005/4
N2 - We assessed the occurrence and screening criterion for extended-spectrum β-lactamases (ESBLs) among AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens. The 413 isolates (158 E. cloacae, 126 C. freundii, and 129 S. marcescens) isolated from 11 clinical laboratories in Korea were investigated. ESBL production was confirmed by double-disk synergy test and inhibitor-potentiated diffusion test using ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZT), and cefepime (FEP) with or without clavulanic acid. One hundred seven isolates (25.9%) were as ESBL producers. Of them, resistance was transferred by conjugation in 82 isolates. In transconjugants, structural genes for CTX-M (53.7%), TEM (46.3%), SHV (29.3%) were found. To evaluate the ESBL screening minimum inhibitory concentration (MIC) criteria, MICs for cefuroxime, CAZ, CTX, AZT, and FEP were determined and cutoff value was selected using receiver operator characteristic curve. The FEP MIC ≥ 1 μg/mL had the highest sensitivity (95.3%), specificity (82.7%), and positive (65.8%) and negative predictive values (98.3%).
AB - We assessed the occurrence and screening criterion for extended-spectrum β-lactamases (ESBLs) among AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens. The 413 isolates (158 E. cloacae, 126 C. freundii, and 129 S. marcescens) isolated from 11 clinical laboratories in Korea were investigated. ESBL production was confirmed by double-disk synergy test and inhibitor-potentiated diffusion test using ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZT), and cefepime (FEP) with or without clavulanic acid. One hundred seven isolates (25.9%) were as ESBL producers. Of them, resistance was transferred by conjugation in 82 isolates. In transconjugants, structural genes for CTX-M (53.7%), TEM (46.3%), SHV (29.3%) were found. To evaluate the ESBL screening minimum inhibitory concentration (MIC) criteria, MICs for cefuroxime, CAZ, CTX, AZT, and FEP were determined and cutoff value was selected using receiver operator characteristic curve. The FEP MIC ≥ 1 μg/mL had the highest sensitivity (95.3%), specificity (82.7%), and positive (65.8%) and negative predictive values (98.3%).
KW - Citrobacter freundii
KW - Enterobacter cloacae
KW - Extended-spectrum β-lactamase
KW - Screening criteria
KW - Serratia marcescens
UR - http://www.scopus.com/inward/record.url?scp=16244389656&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2004.11.009
DO - 10.1016/j.diagmicrobio.2004.11.009
M3 - Article
C2 - 15808318
AN - SCOPUS:16244389656
SN - 0732-8893
VL - 51
SP - 265
EP - 269
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -
